Yuan Ke, Xue Yue-Qin, Yin Ming-Wen, Lou Lu-Huan
Research and Development Certer of Natural Medicine, Zhejiang Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(19):2215-8.
To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time.
The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of methanol-0.05% acetic acid (35:65). The flow rate was 1.0 mL min(-1) and the detection wavelength was set at 340 nm. The column temperature was set at 25 degrees C.
Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2.1%; 101.6%, 2.7%; 98.4%, 2.3%; 99.2%, 1.8%, respectively.
The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.
建立一种反相高效液相色谱法（RP-HPLC），用于同时测定不同产地和采收期淡竹叶中荭草苷、异荭草苷、牡荆素和异牡荆素的含量。
采用HPLC法，色谱柱为Waters XBridge C18柱（4.6 mm×250 mm，5 µm）。流动相为甲醇-0.05%乙酸（35:65）。流速为1.0 mL·min⁻¹，检测波长设定为340 nm。柱温设定为25℃。
四种成分分离良好，线性关系良好。荭草苷、异荭草苷、牡荆素和异牡荆素的平均回收率及相对标准偏差（RSD）分别为103.2%，2.1%；101.6%，2.7%；98.4%，2.3%；99.2%，1.8%。
该HPLC法简便、灵敏、可靠，可用于该药材的质量控制。
