Jones Peter J H, MacKay Dylan S, Senanayake Vijitha K, Pu Shuaihua, Jenkins David J A, Connelly Philip W, Lamarche Benoît, Couture Patrick, Kris-Etherton Penny M, West Sheila G, Liu Xiaoran, Fleming Jennifer A, Hantgan Roy R, Rudel Lawrence L
Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, MB, Canada.
Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, MB, Canada.
Atherosclerosis. 2015 Feb;238(2):231-8. doi: 10.1016/j.atherosclerosis.2014.12.010. Epub 2014 Dec 9.
Oleic acid consumption is considered cardio-protective according to studies conducted examining effects of the Mediterranean diet. However, animal models have shown that oleic acid consumption increases LDL particle cholesteryl oleate content which is associated with increased LDL-proteoglycan binding and atherosclerosis. The objective was to examine effects of varying oleic, linoleic and docosahexaenoic acid consumption on human LDL-proteoglycan binding in a non-random subset of the Canola Oil Multi-center Intervention Trial (COMIT) participants. COMIT employed a randomized, double-blind, five-period, cross-over trial design. Three of the treatment oil diets: 1) a blend of corn/safflower oil (25:75); 2) high oleic canola oil; and 3) DHA-enriched high oleic canola oil were selected for analysis of LDL-proteoglycan binding in 50 participants exhibiting good compliance. LDL particles were isolated from frozen plasma by gel filtration chromatography and LDL cholesteryl esters quantified by mass-spectrometry. LDL-proteoglycan binding was assessed using surface plasmon resonance. LDL particle cholesterol ester fatty acid composition was sensitive to the treatment fatty acid compositions, with the main fatty acids in the treatments increasing in the LDL cholesterol esters. The corn/safflower oil and high-oleic canola oil diets lowered LDL-proteoglycan binding relative to their baseline values (p = 0.0005 and p = 0.0012, respectively). At endpoint, high-oleic canola oil feeding resulted in lower LDL-proteoglycan binding than corn/safflower oil (p = 0.0243) and DHA-enriched high oleic canola oil (p = 0.0249), although high-oleic canola oil had the lowest binding at baseline (p = 0.0344). Our findings suggest that high-oleic canola oil consumption in humans increases cholesteryl oleate percentage in LDL, but in a manner not associated with a rise in LDL-proteoglycan binding.
根据对地中海饮食影响的研究,食用油酸被认为对心脏有保护作用。然而,动物模型表明,食用油酸会增加低密度脂蛋白(LDL)颗粒的胆固醇油酸酯含量,这与LDL-蛋白聚糖结合增加和动脉粥样硬化有关。目的是在菜籽油多中心干预试验(COMIT)参与者的一个非随机子集中,研究不同油酸、亚油酸和二十二碳六烯酸摄入量对人LDL-蛋白聚糖结合的影响。COMIT采用随机、双盲、五阶段交叉试验设计。选择三种治疗油饮食:1)玉米/红花油混合物(25:75);2)高油酸菜籽油;3)富含二十二碳六烯酸的高油酸菜籽油,对50名依从性良好的参与者进行LDL-蛋白聚糖结合分析。通过凝胶过滤色谱法从冷冻血浆中分离LDL颗粒,并通过质谱法定量LDL胆固醇酯。使用表面等离子体共振评估LDL-蛋白聚糖结合。LDL颗粒胆固醇酯脂肪酸组成对治疗脂肪酸组成敏感,治疗中的主要脂肪酸在LDL胆固醇酯中增加。与基线值相比,玉米/红花油和高油酸菜籽油饮食降低了LDL-蛋白聚糖结合(分别为p = 0.0005和p = 0.0012)。在终点时,高油酸菜籽油喂养导致的LDL-蛋白聚糖结合低于玉米/红花油(p = 0.0243)和富含二十二碳六烯酸的高油酸菜籽油(p = 0.0249),尽管高油酸菜籽油在基线时结合最低(p = 0.0344)。我们的研究结果表明,人类食用高油酸菜籽油会增加LDL中胆固醇油酸酯的百分比,但这种方式与LDL-蛋白聚糖结合增加无关。