Fukuchi K, Sakagami H, Okuda T, Hatano T, Tanuma S, Kitajima K, Inoue Y, Inoue S, Ichikawa S, Nonoyama M
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Antiviral Res. 1989 Jun-Jul;11(5-6):285-97. doi: 10.1016/0166-3542(89)90038-7.
Several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (HSV-1, HSV-2)-infected African green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. Among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-HSV activity. Their 50% effective doses (0.03-0.1 microgram/ml) were by two-three orders of magnitude lower than their 50% cytotoxic doses (greater than 10 micrograms/ml). On the other hand, gallic acid, neutral polysaccharides, chemically modified (N,N-dimethylaminoethyl-, carboxymethyl-, and sulfated-) glucans, sialic acid-rich glycoproteins, and uronic acid-rich pine cone polysaccharide showed little or no activity. Using radiolabeled virus particles, we demonstrated that the anti-HSV effect of the tannins is due to inhibition of virus adsorption to the cells.
使用蚀斑形成试验，研究了几种化学成分明确的植物提取物对感染单纯疱疹病毒（HSV - 1、HSV - 2）的非洲绿猴肾细胞和人腺癌细胞的抗病毒作用。其中，具有没食子酰基或六羟基二苯甲酰基的单体可水解单宁、低聚鞣花单宁和缩合单宁具有最强的抗HSV活性。它们的50%有效剂量（0.03 - 0.1微克/毫升）比50%细胞毒性剂量（大于10微克/毫升）低两到三个数量级。另一方面，没食子酸、中性多糖、化学修饰（N，N - 二甲基氨基乙基 -、羧甲基 - 和硫酸化 -）葡聚糖、富含唾液酸的糖蛋白以及富含糖醛酸的松果多糖显示出很少或没有活性。使用放射性标记的病毒颗粒，我们证明了单宁的抗HSV作用是由于抑制病毒吸附到细胞上。
