Lopes Nayara, Ray Sayani, Espada Samantha Fernandes, Bomfim Weslei Andrade, Ray Bimalendu, Faccin-Galhardi Lígia Carla, Linhares Rosa Elisa Carvalho, Nozawa Carlos
Departamento de Microbiologia, CCB, Universidade Estadual de Londrina, CEP 86051-990 Londrina, Paraná, Brazil.
Department of Chemistry, University of Burdwan, Bardhaman, West Bengal, India.
Int J Biol Macromol. 2017 Sep;102:605-612. doi: 10.1016/j.ijbiomac.2017.04.043. Epub 2017 Apr 19.
The herpes simplex virus (HSV) diseases represent a relevant medical and social problem due to their communicability and recurrence following latency. The green algae are rich source of polysaccharides referred to as ulvans, reported as being biologically and pharmacologically active. In this work, we analyzed the activity of seven chemically modified polysaccharides from Enteromorpha compressa (Chlorophyta, Ulvaceae), against HSV. Only the derivative named SU1F1 showed satisfactory viral inhibition activity, with a high selectivity index, and, therefore, it was submitted to analysis of the probable mechanism of action and structure. SU1F1 is a sulphated (22% w/w) heteroglycuronan with an apparent molecular mass of 34kDa. The antiviral activity was assayed by plaque reduction assay under the protocols of the time-of-addition (from 3h before infection to 16h after infection), the inhibition of virus adsorption and penetration, and the virucidal effects. SU1F1 showed a high viral activity at the time 0h. We demonstrated that its inhibitory effect was maintained until 4h post-treatment with 100% of viral inhibition at 100μg/ml. No effect was observed in additional protocols (the pre-treatment, the inhibition of adsorption and penetration and virucidal assays). Reverse Transcriptase associated PCR (RT-PCR) results were in accordance with plaque reduction assay and demonstrated the activity of SU1F1 at the initial stages of HSV replication.
单纯疱疹病毒（HSV）疾病因其传染性和潜伏后的复发性而成为一个重要的医学和社会问题。绿藻是多糖的丰富来源，这些多糖被称为石莼多糖，据报道具有生物和药理活性。在这项工作中，我们分析了七种来自浒苔（绿藻门，石莼科）的化学修饰多糖对HSV的活性。只有名为SU1F1的衍生物表现出令人满意的病毒抑制活性，具有高选择性指数，因此，对其可能的作用机制和结构进行了分析。SU1F1是一种硫酸化（22% w/w）的杂聚葡糖醛酸聚糖，表观分子量为34kDa。通过蚀斑减少试验，按照添加时间（从感染前3小时到感染后16小时）、病毒吸附和穿透抑制以及杀病毒作用的方案来测定抗病毒活性。SU1F1在0小时时显示出高病毒活性。我们证明其抑制作用一直维持到治疗后4小时，在100μg/ml时病毒抑制率为100%。在其他方案（预处理、吸附和穿透抑制以及杀病毒试验）中未观察到效果。逆转录酶相关PCR（RT-PCR）结果与蚀斑减少试验一致，并证明了SU1F1在HSV复制初始阶段的活性。
