Malouli Daniel, Howell Grant L, Legasse Alfred W, Kahl Christoph, Axthelm Michael K, Hansen Scott G, Früh Klaus
Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America.
Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, United States of America.
Virol Rep. 2014 Sep;3-4:18-29. doi: 10.1016/j.virep.2014.10.001.
Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genus containing only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.
在过去几年中，已分离出多种新型猿猴腺病毒，它们跨越物种屏障并感染人类的可能性始终是一个威胁。在此，我们描述了从两只独立饲养、从未共同饲养且处于晚期猿猴免疫缺陷病毒（SIV）感染状态的恒河猴尿液中分离出的一种新型猿猴腺病毒（SAdV）及其全基因组测序情况。病毒基因组序列显示这是一种新型病毒，具有独特的基因组长度、GC含量、E3区域和DNA聚合酶氨基酸序列，与目前所有已知的人类或猿猴腺病毒种类有足够差异，足以将这些分离株归类为一种新型猿猴腺病毒。这个新物种被命名为猿猴mastadenovirus D（SAdV-D），它展示了该属的标准基因组结构，仅含有一个纤维基因拷贝，这使其有别于旧世界猴腺病毒种类HAdV-G、SAdV-B和SAdV-C。
