Jing Lei, Wang Yun, Zhao Xiao-Min, Zhao Bing, Han Ji-Ju, Qin Shu-Cun, Sun Xue-Jun
Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China; Department of Pharmocology, Taishan Medical University, Taian, 271000, China; Department of Pharmacy, Dongping County People's Hospital, Dongping, 271500, China.
Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China.
Heart Lung Circ. 2015 Jun;24(6):602-10. doi: 10.1016/j.hlc.2014.11.018. Epub 2014 Dec 4.
Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant and anti-inflammatory activity that may protect against organ damage induced by oxidative stress. Therefore because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of our study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats.
An acute MI model was induced in male Wistar rats by subcutaneous injection of isoproterenol. Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats. Oxidative stress indices including levels of myocardial marker enzymes, inflammatory cytokines, membrane-bound myocardial enzymes and histopathological changes were measured.
Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished infarct size, improved left heart function, and ameliorated pathological changes of the left heart.
From these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities.
最近有报道称，输注氢气饱和生理盐水具有抗氧化和抗炎活性，可能预防氧化应激诱导的器官损伤。因此，鉴于氧化应激在心肌梗死（MI）的病理生理学中起重要作用，我们研究的目的是探讨富氢生理盐水对异丙肾上腺素诱导的大鼠心肌梗死是否具有心脏保护作用。
通过皮下注射异丙肾上腺素在雄性Wistar大鼠中诱导急性心肌梗死模型。给大鼠腹腔注射不同剂量的富氢生理盐水（5、7.5和10 mL/kg体重）或口服维生素C（250 mg/kg体重）。测量氧化应激指标，包括心肌标志物酶水平、炎性细胞因子、膜结合心肌酶和组织病理学变化。
与异丙肾上腺素诱导心肌梗死组相比，富氢生理盐水降低了丙二醛和8-羟基脱氧鸟苷浓度，增强了超氧化物歧化酶和Na(+)-K(+)-ATP酶活性，降低了Ca(2+)-ATP酶活性，并降低了大鼠血清和/或心脏组织中白细胞介素-6和肿瘤坏死因子-α水平。富氢生理盐水预处理还减小了梗死面积，改善了左心功能，并减轻了左心的病理变化。
从这些结果来看，富氢生理盐水至少部分通过引发抗氧化和抗炎活性的相互作用，对异丙肾上腺素诱导的心肌梗死发挥心血管保护作用。
