Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
Department of Entomology, S-225 Agricultural Science Center North, University of Kentucky Lexington, KY, USA.
Front Physiol. 2014 Dec 10;5:487. doi: 10.3389/fphys.2014.00487. eCollection 2014.
Host-associated differentiation is one of the driving forces behind the diversification of phytophagous insects. In this study, host induced transcriptomic differences were investigated in the sweetpotato whitefly Bemisia tabaci, an invasive agricultural pest worldwide. Comparative transcriptomic analyses using coding sequence (CDS), 5' and 3' untranslated regions (UTR) showed that sequence divergences between the original host plant, cabbage, and the derived hosts, including cotton, cucumber and tomato, were 0.11-0.14%, 0.19-0.26%, and 0.15-0.21%, respectively. In comparison to the derived hosts, 418 female and 303 male transcripts, respectively, were up-regulated in the original cabbage strain. Among them, 17 transcripts were consistently up-regulated in both female and male whiteflies originated from the cabbage host. Specifically, two ESTs annotated as Cathepsin B or Cathepsin B-like genes were significantly up-regulated in the original cabbage strain, representing a transcriptomic response to the dietary challenges imposed by the host shifting. Results from our transcriptome analysis, in conjunction with previous reports documenting the minor changes in their reproductive capacity, insecticide susceptibility, symbiotic composition and feeding behavior, suggest that the impact of host-associated differentiation in whiteflies is limited. Furthermore, it is unlikely the major factor contributing to their rapid range expansion/invasiveness.
宿主相关分化是植食性昆虫多样化的驱动力之一。本研究调查了全世界农业害虫烟粉虱（Bemisia tabaci）在取食不同宿主植物时的转录组差异。通过对编码序列（CDS）、5' 和 3' 非翻译区（UTR）的比较转录组分析发现，与原始宿主植物（甘蓝）相比，衍生宿主（棉花、黄瓜和番茄）间的序列差异分别为 0.11-0.14%、0.19-0.26%和 0.15-0.21%。与衍生宿主相比，在原始甘蓝种群中，分别有 418 个雌性和 303 个雄性转录本上调。其中，有 17 个转录本在取食甘蓝的雌雄烟粉虱中均持续上调。特别地，两个注释为组织蛋白酶 B 或组织蛋白酶 B 样基因的 EST 在原始甘蓝种群中显著上调，这代表了烟粉虱对取食不同宿主的转录组响应。本转录组分析结果与先前关于其生殖能力、杀虫剂敏感性、共生组成和取食行为变化较小的报道相结合，表明宿主相关分化对白粉虱的影响有限，不太可能是其快速扩散/入侵的主要因素。
