Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, USA.
Trends Biotechnol. 2015 Feb;33(2):111-9. doi: 10.1016/j.tibtech.2014.11.009. Epub 2014 Dec 24.
The ability to link systems together such that they behave as predicted once they interact with each other is an essential requirement for the forward-engineering of robust synthetic biological circuits. Unfortunately, because of context-dependencies, parts and functional modules often behave unpredictably once interacting in the cellular environment. This paper reviews recent advances toward establishing a rigorous engineering framework for insulating parts and modules from their context to improve modularity. Overall, a synergy between engineering better parts and higher-level circuit design will be important to resolve the problem of context-dependence.
能够将系统连接在一起，使它们在相互作用后表现出预期的行为，这是正向工程构建稳健的合成生物电路的基本要求。不幸的是，由于上下文相关性，部件和功能模块在细胞环境中相互作用时常常表现出不可预测的行为。本文综述了在建立严格的工程框架以将部件和模块与其上下文隔离开来提高模块性方面的最新进展。总的来说，改进部件和更高层次的电路设计之间的协同作用对于解决上下文相关性问题将是重要的。
