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轮状病毒亚组特异性单克隆抗体的特性及其在单夹心ELISA系统中用于人毒株快速亚组分型的应用

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.

作者信息

Gerna G, Sarasini A, Torsellini M, di Matteo A, Baldanti F, Parea M, Battaglia M

机构信息

Virus Laboratory, University of Pavia, Italy.

出版信息

Arch Virol. 1989;107(3-4):315-22. doi: 10.1007/BF01317927.

DOI:10.1007/BF01317927
PMID:2554860
Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

摘要

两种在小鼠体内产生的针对A组人轮状病毒的亚组特异性单克隆抗体，经免疫印迹法显示可与同源亚组VP6的三聚体形式发生反应，并成功应用于开发新的单夹心ELISA系统，用于对人毒株进行快速亚组分型。所检测的344株病毒均可进行亚组分型，但其中两株需要事先在细胞培养物中传代。

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1
Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.轮状病毒亚组特异性单克隆抗体的特性及其在单夹心ELISA系统中用于人毒株快速亚组分型的应用
Arch Virol. 1989;107(3-4):315-22. doi: 10.1007/BF01317927.
2
Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination.抗人轮状病毒亚组特异性单克隆抗体的制备及其在酶联免疫吸附测定法中用于亚组测定的应用。
J Med Virol. 1984;14(2):115-25. doi: 10.1002/jmv.1890140205.
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J Clin Microbiol. 1988 Jun;26(6):1238-40. doi: 10.1128/jcm.26.6.1238-1240.1988.
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An equine rotavirus (FI-14 strain) which bears both subgroup I and subgroup II specificities on its VP6.一种马轮状病毒（FI-14株），其VP6上兼具I亚组和II亚组特异性。
Virology. 1987 Apr;157(2):488-96. doi: 10.1016/0042-6822(87)90291-1.
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Monoclonal antibodies to the VP6 of porcine subgroup I rotaviruses reactive with subgroup I and non-subgroup I non-subgroup II strains.针对猪I群轮状病毒VP6的单克隆抗体，可与I群以及非I群非II群毒株发生反应。
J Gen Virol. 1990 Jun;71 ( Pt 6):1395-8. doi: 10.1099/0022-1317-71-6-1395.
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J Virol Methods. 1989 Apr-May;24(1-2):43-56. doi: 10.1016/0166-0934(89)90006-2.
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Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7.通过使用针对VP7的1型、2型、3型和4型特异性单克隆抗体的酶联免疫吸附测定法对粪便中的人轮状病毒进行直接血清分型。
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Virology. 1992 Nov;191(1):272-81. doi: 10.1016/0042-6822(92)90189-v.

引用本文的文献

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Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype.在欧洲分离出69株具有I或II亚组特异性以及长RNA电泳型的M样（血清型8）人轮状病毒毒株。
Arch Virol. 1990;112(1-2):27-40. doi: 10.1007/BF01348983.
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Isolation and characterization of two distinct human rotavirus strains with G6 specificity.两种具有G6特异性的不同人类轮状病毒株的分离与鉴定。
J Clin Microbiol. 1992 Jan;30(1):9-16. doi: 10.1128/jcm.30.1.9-16.1992.

本文引用的文献

1
Relative frequency of rotavirus subgroups 1 and 2 in Venezuelan children with gastroenteritis as assayed with monoclonal antibodies.用单克隆抗体检测委内瑞拉患肠胃炎儿童中轮状病毒1型和2型的相对频率。
J Clin Microbiol. 1984 Apr;19(4):516-20. doi: 10.1128/jcm.19.4.516-520.1984.
2
Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization.通过中和作用对细胞培养适应的2型人轮状病毒株进行血清分型
Infect Immun. 1984 Feb;43(2):722-9. doi: 10.1128/iai.43.2.722-729.1984.
3
Detection of a human rotavirus strain different from types 1 and 2--a new subgroup? Epidemiology of subgroups in a Swedish and an Ethiopian community.
一种不同于1型和2型的人轮状病毒株的检测——一个新的亚群？瑞典和埃塞俄比亚社区中亚群的流行病学。
J Med Virol. 1983;12(2):111-7. doi: 10.1002/jmv.1890120205.
4
Comparison of immunosorbent electron microscopy, enzyme immunoassay and counterimmunoelectrophoresis for detection of human rotavirus in stools.免疫吸附电子显微镜法、酶免疫测定法和对流免疫电泳法检测粪便中人类轮状病毒的比较
J Virol Methods. 1981 Sep;3(2):99-107. doi: 10.1016/0166-0934(81)90006-9.
5
Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens.编码中和及亚组抗原的人（Wa株）和牛（UK株）轮状病毒基因。
Virology. 1981 Jul 30;112(2):385-90. doi: 10.1016/0042-6822(81)90285-3.
6
Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.用于检测儿童肠胃炎病毒的直接电子显微镜检查、免疫电子显微镜检查和轮状病毒酶联免疫吸附测定的比较
J Clin Microbiol. 1981 May;13(5):976-81. doi: 10.1128/jcm.13.5.976-981.1981.
7
Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.通过蚀斑减少中和试验研究的哺乳动物和禽类来源轮状病毒的血清型相似性和多样性
J Infect Dis. 1984 May;149(5):694-702. doi: 10.1093/infdis/149.5.694.
8
Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies.使用单克隆抗体对轮状病毒亚组蛋白进行血清学分析。
Infect Immun. 1983 Jan;39(1):91-9. doi: 10.1128/iai.39.1.91-99.1983.
9
Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination.抗人轮状病毒亚组特异性单克隆抗体的制备及其在酶联免疫吸附测定法中用于亚组测定的应用。
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Subgrouping of human rotavirus strains by complement fixation, indirect double-antibody sandwich enzyme-linked immunosorbent assay and solid-phase immune electron microscopy.通过补体结合试验、间接双抗体夹心酶联免疫吸附测定和固相免疫电镜对人轮状病毒毒株进行亚群分类。
Arch Virol. 1984;81(3-4):193-203. doi: 10.1007/BF01309992.