Ma Ke, Yan Naihong, Huang Yongzhi, Cao Guiqun, Deng Jie, Deng Yingping
Department of Ophthalmology and Ophthalmic Laboratories, West China Hospital, Sichuan University Chengdu, China.
Int J Clin Exp Med. 2014 Nov 15;7(11):4584-9. eCollection 2014.
The study aims to determine the relation between the effects of mouse nerve growth factor (mNGF) and nerve regeneration after corneal surgery nerve damage. Mechanical nerve injury animal model was established by LASIK (the excimer laser keratomileusis) surgery in 12 Belgian rabbits. mNGF and the balanced salt solution (BBS) were alternatively administered in the left and right eye two times every day for 8 weeks. The morphous and growth of the sub-basal nerve plexus and superficial stroma were observed by in vivo confocal microscopy at the end of weeks 1, 2, 4 and 8 after the surgery. The animal model is successfully established. The morphology and density of corneal nerve have been observed and demonstrated by confocal microscopy. A systematic administration of mNGF can significantly promote the nerve regeneration at the end of weeks 1, 2, 4 and 8, which comparing to the administration of balanced salt solution (P < 0.05). mNGF has effect on sub-basal nerve plexus and superficial stroma after corneal nerve damage which is caused by LASIK. The experimental results suggested that the mNGF may solve the problem of dry eye after LASIK.
本研究旨在确定小鼠神经生长因子（mNGF）的作用与角膜手术神经损伤后神经再生之间的关系。通过准分子激光原位角膜磨镶术（LASIK）对12只比利时兔进行手术，建立机械性神经损伤动物模型。每天两次交替在左眼和右眼给予mNGF和平衡盐溶液（BBS），持续8周。在术后第1、2、4和8周结束时，通过活体共聚焦显微镜观察角膜基底膜下神经丛和浅层基质的形态及生长情况。动物模型成功建立。通过共聚焦显微镜观察并证实了角膜神经的形态和密度。与给予平衡盐溶液相比，系统性给予mNGF在第1、2、4和8周结束时可显著促进神经再生（P < 0.05）。mNGF对LASIK所致角膜神经损伤后的角膜基底膜下神经丛和浅层基质有作用。实验结果表明，mNGF可能解决LASIK术后干眼问题。
