Ryseck R P, Macdonald-Bravo H, Mattéi M G, Ruppert S, Bravo R
European Molecular Biology Laboratory, Heidelberg, FRG.
EMBO J. 1989 Nov;8(11):3327-35. doi: 10.1002/j.1460-2075.1989.tb08494.x.
We have characterized a growth factor inducible gene, N10, encoding a nuclear protein of 601 amino acids with a significant similarity to members of the steroid and thyroid hormone receptor families. The gene is rapidly but transiently induced by several mitogens. Immunoprecipitation studies show that the N10 protein is transiently expressed after stimulation of quiescent cells, presenting a half-life of approximately 30 min. The N10 transcription unit is 8 kb in length, split into seven exons. The exon-intron distribution is in general similar to that of other members of the nuclear receptor superfamily, but presents some differences which suggest that N10 belongs to a new family of these molecules. The 5' flanking region contains one DSE which could explain its immediate response to external stimulus. The N10 gene is located in the [F1-F3] region of mouse chromosome 15.
我们已对一种生长因子诱导基因N10进行了特征描述，该基因编码一种由601个氨基酸组成的核蛋白，与类固醇和甲状腺激素受体家族成员具有显著相似性。该基因可被多种有丝分裂原快速但短暂地诱导。免疫沉淀研究表明，静止细胞受到刺激后，N10蛋白会短暂表达，其半衰期约为30分钟。N10转录单元长度为8 kb，分为七个外显子。外显子-内含子分布总体上与核受体超家族的其他成员相似，但存在一些差异，这表明N10属于这些分子的一个新家族。5'侧翼区域包含一个DSE，这可以解释其对外部刺激的即时反应。N10基因位于小鼠15号染色体的[F1-F3]区域。
