Gupta S, Malviya N, Kushwaha H, Nasim J, Bisht N C, Singh V K, Yadav D
Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, 273 009, Uttar Pradesh, India.
Planta. 2015 Mar;241(3):549-62. doi: 10.1007/s00425-014-2239-3. Epub 2015 Jan 7.
The structural, functional and in-silico studies of Dof transcription factor attempted so far reveals immense opportunity to analyze the plant genomes in terms of number of Dof genes and discuss in light of the evolution. The multiple functions of Dof genes needs to explored for crop improvement. Transcription factors play a very vital role in gene regulation at transcriptional level and are being extensively studied across phylas. In recent years, sequencing of plant genomes has led to genome-wide identification and characterizations of diverse types of plant-specific transcription factor gene family providing key insights into their structural and functional diversity. The DNA binding with one finger (Dof), a class belonging to C2H2-type zinc finger family proteins, is a plant-specific transcription factor having multiple roles such as seed maturation and germination, phytohormone and light-mediated regulation and plant responses to biotic and abiotic stresses. Dof proteins are present across plant lineage, from green algae to higher angiosperm, and represent a unique class of transcription factor having bifunctional binding activities, with both DNA and proteins, to regulate the complex transcriptional machinery in plant cells. The structural and functional diversity of the Dof transcription factor family along with the bioinformatics analysis highlighting the phylogeny of Dof families is reviewed in light of its importance in plant biotechnology for crop improvement.
迄今为止，对Dof转录因子进行的结构、功能及计算机模拟研究揭示了从Dof基因数量方面分析植物基因组并结合进化进行讨论的巨大机会。Dof基因的多种功能有待探索以用于作物改良。转录因子在转录水平的基因调控中起着至关重要的作用，并且正在跨门类进行广泛研究。近年来，植物基因组测序已导致全基因组范围内对多种类型植物特异性转录因子基因家族的鉴定和表征，为其结构和功能多样性提供了关键见解。单指DNA结合（Dof）属于C2H2型锌指家族蛋白，是一种植物特异性转录因子，具有多种作用，如种子成熟和萌发、植物激素和光介导的调控以及植物对生物和非生物胁迫的响应。Dof蛋白存在于从绿藻到高等被子植物的整个植物谱系中，代表一类独特的转录因子，具有与DNA和蛋白质的双功能结合活性，以调节植物细胞中的复杂转录机制。鉴于Dof转录因子家族在植物生物技术中对作物改良的重要性，本文对其结构和功能多样性以及突出Dof家族系统发育的生物信息学分析进行了综述。
