Ma Jing, Li Meng-Yao, Wang Feng, Tang Jun, Xiong Ai-Sheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
BMC Genomics. 2015 Jan 31;16(1):33. doi: 10.1186/s12864-015-1242-9.
Chinese cabbage is an important leaf vegetable that experienced long-term cultivation and artificial selection. Dof (DNA-binding One Zinc Finger) transcription factors, with a highly conserved Dof domain, are members of a major plant-specific transcription factor family that play important roles in many plant biological processes. The Dof family transcription factors, one of the most important families of transcriptional regulators in higher plants, are involved in massive aspects of plant growth, development, and response to abiotic stresses. Our study will supply resources for understanding how Dof transcription factors respond to abiotic stress and the interaction network of these genes in tolerance mechanism.
In this study, we performed a comprehensive analysis of Dof family factors in Chinese cabbage. In total, 76 genes encoding BraDof family transcription factor were identified from Chinese cabbage, and those BraDof factors were divided into nine classes. Fifteen motifs were found based on Dof amino acid sequence alignments. Chromosome locations and gene duplications of BraDof family genes were also analyzed. Ten duplicate events of BraDof genes were discovered in Chinese cabbage chromosomes. The uneven distribution of BraDof genes in Brassica chromosomes may cause the expansion of BraDof genes. In the Dof family, 37 and 7 orthologous genes were identified between Chinese cabbage and Arabidopsis and between Chinese cabbage and Oryza sativa, respectively. The interaction networks of Dof factors in Chinese cabbage were also constructed. Expression profiles of nine selected genes from different nine classes subjected to four abiotic stresses (cold, heat, salt and drought) were further investigated by quantitative real-time PCR to obtain a better understanding of the functions and regulation mechanisms of BraDof family transcription factors in two Chinese cabbage varieties, 'Lubaisanhao' and 'Qingdao 87-114'.
Dof-family transcription factors were analyzed in genome of Chinese cabbage. Chromosomal locations showed that duplication might result in expansion. Response to abiotic stresses was elucidated in Chinese cabbage varieties. The results provide novel insights into the stress responses of BraDof genes and promote a better understanding of the construction and function of Dofs in Chinese cabbage.
大白菜是一种经过长期栽培和人工选择的重要叶菜类蔬菜。Dof（DNA结合单锌指）转录因子具有高度保守的Dof结构域，是植物特有的主要转录因子家族成员，在许多植物生物学过程中发挥重要作用。Dof家族转录因子是高等植物中最重要的转录调节因子家族之一，参与植物生长、发育以及对非生物胁迫响应的诸多方面。我们的研究将为理解Dof转录因子如何响应非生物胁迫以及这些基因在耐受机制中的相互作用网络提供资源。
在本研究中，我们对大白菜中的Dof家族因子进行了全面分析。从大白菜中总共鉴定出76个编码BraDof家族转录因子的基因，这些BraDof因子被分为九类。基于Dof氨基酸序列比对发现了15个基序。还分析了BraDof家族基因的染色体定位和基因复制情况。在大白菜染色体中发现了10个BraDof基因的重复事件。BraDof基因在芸苔属染色体中的不均匀分布可能导致了BraDof基因的扩增。在Dof家族中，分别在大白菜与拟南芥以及大白菜与水稻之间鉴定出37个和7个直系同源基因。还构建了大白菜中Dof因子的相互作用网络。通过定量实时PCR进一步研究了从不同九类中选出的9个基因在四种非生物胁迫（冷、热、盐和干旱）下的表达谱，以更好地了解BraDof家族转录因子在两个大白菜品种‘鲁白三号’和‘青岛87 - 114’中的功能和调控机制。
对大白菜基因组中的Dof家族转录因子进行了分析。染色体定位表明复制可能导致扩增。阐明了大白菜品种对非生物胁迫的响应。这些结果为BraDof基因的胁迫响应提供了新见解，并有助于更好地理解大白菜中Dof的结构和功能。
