Chen Aie, Zheng Weiwei, Zheng Wenping, Zhang Hongyu
State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control and Institute of Urban and Horticultural Pests, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Ecotoxicology. 2015 Apr;24(3):595-603. doi: 10.1007/s10646-014-1407-3. Epub 2015 Jan 9.
Double-stranded RNA (dsRNA) designed to target pest genes emerges as a promising strategy for improving pest control. Therefore, it is necessary to assess the effects of dsRNA on non-target insects, such as native enemies and beneficial insects, to determine the environmental safety of such treatments. In this paper, we investigated the effects of dsRNA targeting rpl19 from Bactrocera dorsalis on non-target insects in citrus ecological systems by feeding the dsRNA to Bactrocera minax, Apis mellifera and Diachasmimorpha longicaudata. The results showed that when B. dorsalis were fed rpl19 CDS dsRNA or 3'UTR dsRNA, the expression of rpl19 was dramatically decreased. Feeding the Bdrpl19 CDS dsRNA to adult B. minax and D. longicaudata caused their respective rpl19 genes to be knocked down over 50-70 and 40%, respectively, but it had no effect on the expression of the rpl19 gene in A. mellifera. The Bdrpl19 3'UTR dsRNA did not have any silencing effects on the expression levels of rpl19 in non-target insects. This study provides evidence that dsRNA can impact non-target organisms, but the 3'UTR dsRNA may not have effects in non-target organisms.
旨在靶向害虫基因的双链RNA（dsRNA）已成为一种有前景的害虫防治策略。因此，有必要评估dsRNA对非靶标昆虫（如天敌和益虫）的影响，以确定此类处理的环境安全性。在本文中，我们通过向橘小实蝇、意大利蜜蜂和长尾潜蝇茧蜂投喂dsRNA，研究了靶向橘小实蝇rpl19的dsRNA对柑橘生态系统中非靶标昆虫的影响。结果表明，当给橘小实蝇投喂rpl19 CDS dsRNA或3'UTR dsRNA时，rpl19的表达显著降低。向成年橘小实蝇和长尾潜蝇茧蜂投喂Bdrpl19 CDS dsRNA分别导致它们各自的rpl19基因敲低超过50%-70%和40%，但对意大利蜜蜂中rpl19基因的表达没有影响。Bdrpl19 3'UTR dsRNA对非靶标昆虫中rpl19的表达水平没有任何沉默作用。本研究提供了证据表明dsRNA会影响非靶标生物，但3'UTR dsRNA可能对非靶标生物没有影响。
