Li Jialin, Xie Jiale, Gao Lixia, Li Chang Ming
Institute for Clean Energy & Advanced Materials and ‡Faculty of Materials and Energy, Southwest University , Chongqing 400715, China.
ACS Appl Mater Interfaces. 2015 Feb 4;7(4):2726-34. doi: 10.1021/am5077777. Epub 2015 Jan 22.
In situ detection of nitric oxide (NO) released from living cells has become very important in studies of some critical physiological and pathological processes, but it is still very challenging due to the low concentration and fast decay of NO. A nanocomposite of Au nanoparticles deposited on three-dimensional graphene hydrogel (Au NPs-3DGH) was prepared through a facile one-step approach by in situ reduction of Au(3+) on 3DGH to build a unique sensing film for a strong synergistic effect, in which the highly porous 3DGH offers a large surface area while Au NPs uniformly deposited on 3DGH efficiently catalyze the electrochemical oxidation of NO for sensitive detection of NO with excellent selectivity, fast response, and low detection limit. The sensor was further used to in situ detect NO released from living cells under drug stimulation, showing significant difference between normal and tumor cells under drug stimulation.
在一些关键生理和病理过程的研究中，对活细胞释放的一氧化氮（NO）进行原位检测变得非常重要，但由于NO浓度低且衰减快，这仍然极具挑战性。通过一种简便的一步法，在三维石墨烯水凝胶（3DGH）上原位还原Au(3+)，制备了一种沉积有金纳米颗粒的纳米复合材料（Au NPs-3DGH），以构建具有强协同效应的独特传感膜。其中，高度多孔的3DGH提供了大表面积，而均匀沉积在3DGH上的Au NPs有效地催化NO的电化学氧化，从而实现对NO的灵敏检测，具有出色的选择性、快速响应和低检测限。该传感器进一步用于原位检测药物刺激下活细胞释放的NO，显示出药物刺激下正常细胞和肿瘤细胞之间的显著差异。
