Laiho K
Department of Forensic Medicine, University of Helsinki, Finland.
Z Rechtsmed. 1989;103(2):101-9. doi: 10.1007/BF01258913.
A method is described for the removal of contaminating hemoglobin from the peroxidase enzyme in traumatic skin lesions. The procedure is based on hemoglobin precipitation in a combination of ammonium sulfate half-saturation, and chloroform shaking of the cetyltrimethylammonium-bromide extract. The procedure as such somewhat increases the activity of the peroxidase extract if the extract contains no hemoglobin. On the other hand, the peroxidase activity of the extract decreases as the amount of precipitating hemoglobin increases. On average, about 90% of the peroxidase activity persists after hemoglobin precipitation if the hemoglobin concentration in the extract does not exceed 25 mg/100 ml. In experimental incision wounds, the peroxidase activities obtained with this procedure were the same as when enzyme determinations were done without the removal of hemoglobin or slightly higher. In addition, the amount of peroxidase activity in the wounds was estimated, based on the granulocytes of the contaminating blood.
本文描述了一种从创伤性皮肤损伤中的过氧化物酶去除污染血红蛋白的方法。该方法基于在硫酸铵半饱和以及十六烷基三甲基溴化铵提取物的氯仿振荡联合作用下血红蛋白的沉淀。如果提取物不含血红蛋白，该方法本身会在一定程度上提高过氧化物酶提取物的活性。另一方面，随着沉淀血红蛋白量的增加，提取物的过氧化物酶活性降低。如果提取物中的血红蛋白浓度不超过25mg/100ml，平均而言，血红蛋白沉淀后约90%的过氧化物酶活性得以保留。在实验性切口伤口中，采用该方法获得的过氧化物酶活性与未去除血红蛋白时进行酶测定的结果相同或略高。此外，还基于污染血液中的粒细胞对伤口中的过氧化物酶活性量进行了估计。
