Coconnier M H, Klaenhammer T R, Kernéis S, Bernet M F, Servin A L
Département de Microbiologie et Immunologie, UFR Sciences Pharmaceutiques Paris XI, Chatenay-Malabry, France.
Appl Environ Microbiol. 1992 Jun;58(6):2034-9. doi: 10.1128/aem.58.6.2034-2039.1992.
The adhesion of Lactobacillus acidophilus BG2FO4, a human stool isolate, to two human enterocytelike cell lines (Caco-2 and HT-29) and to the mucus secreted by a subpopulation of mucus-secreting HT29-MTX cells was investigated. Scanning electron microscopy revealed that the bacteria interacted with the well-defined apical microvilli of Caco-2 cells without cell damage and with the mucus secreted by the subpopulation of HT29-MTX cells. The adhesion to Caco-2 cells did not require calcium and involved an adhesion-promoting factor that was present in the spent supernatant of L. acidophilus cultures. This factor promoted adhesion of poorly adhering human Lactobacillus casei GG but did not promote adhesion of L. casei CNRZ 387, a strain of dairy origin. The adherence components on the bacterial cells and in the spent supernatant were partially characterized. Carbohydrates on the bacterial cell wall appeared to be partly responsible for the interaction between the bacteria and the extracellular adhesion-promoting factor. The adhesion-promoting factor was proteinaceous, since trypsin treatment dramatically decreased the adhesion of the L. acidophilus strain. The adhesion-promoting factor may be an important component of Lactobacillus species that colonize the gastrointestinal tract.
对从人类粪便中分离出的嗜酸乳杆菌BG2FO4与两种人类肠样细胞系（Caco-2和HT-29）以及HT29-MTX黏液分泌亚群分泌的黏液之间的黏附情况进行了研究。扫描电子显微镜显示，该细菌与Caco-2细胞界限分明的顶端微绒毛相互作用且未造成细胞损伤，同时也与HT29-MTX细胞亚群分泌的黏液相互作用。对Caco-2细胞的黏附不需要钙，且涉及嗜酸乳杆菌培养物的用过的上清液中存在的一种黏附促进因子。该因子促进了黏附性较差的干酪乳杆菌GG的黏附，但不促进源自乳制品的干酪乳杆菌CNRZ 387的黏附。对细菌细胞和用过的上清液中的黏附成分进行了部分表征。细菌细胞壁上的碳水化合物似乎部分负责细菌与细胞外黏附促进因子之间的相互作用。黏附促进因子是蛋白质类的，因为胰蛋白酶处理显著降低了嗜酸乳杆菌菌株的黏附。黏附促进因子可能是定殖于胃肠道的乳杆菌属物种的一个重要组成部分。
