HDRsEf1保护肠道上皮并减弱肠毒素大肠杆菌诱导的肠细胞中白细胞介素-8的分泌。

HDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes.

作者信息

Tian Zhongyuan, Liu Xiaofang, Dai Ran, Xiao Yuncai, Wang Xiliang, Bi Dingren, Shi Deshi

机构信息

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.

College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China.

出版信息

Mediators Inflamm. 2016;2016:7474306. doi: 10.1155/2016/7474306. Epub 2016 Nov 7.

The probiotic HDRsEf1 (Ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated. In this study, using the IPEC-J2 cell line to mimic intestinal epithelial cells and enterotoxigenic (ETEC) K88ac as a representative intestinal pathogen, the mechanism underlying Ef1 protection against an enteropathogen was investigated. The results demonstrated that Ef1 was effective in displacing K88ac from the IPEC-J2 cell layer. Moreover, Ef1 and its cell-free supernatant (S-Ef1) modulate IL-8 released by IPEC-J2 cells. Ef1 and its cell-free supernatant showed the potential to protect enterocytes from an acute inflammatory response. In addition, Ef1 and its cell-free supernatant increased the transepithelial electrical resistance (TEER) of the enterocyte monolayer, thus strengthening the intestinal barrier against ETEC. These results may contribute to the development of therapeutic interventions using Ef1 in intestinal disorders of piglets.

益生菌HDRsEf1（Ef1）已被证明对仔猪腹泻有积极作用，但其机制尚未阐明。在本研究中，使用IPEC-J2细胞系模拟肠上皮细胞，并以产肠毒素大肠杆菌（ETEC）K88ac作为代表性肠道病原体，研究了Ef1对肠道病原体的保护机制。结果表明，Ef1能有效地将K88ac从IPEC-J2细胞层中置换出来。此外，Ef1及其无细胞上清液（S-Ef1）可调节IPEC-J2细胞释放的IL-8。Ef1及其无细胞上清液显示出保护肠上皮细胞免受急性炎症反应的潜力。此外，Ef1及其无细胞上清液增加了肠上皮细胞单层的跨上皮电阻（TEER），从而加强了肠道对ETEC的屏障。这些结果可能有助于开发利用Ef1治疗仔猪肠道疾病的干预措施。