Yang Song, Lu Mingdian, Chen Yuanyuan, Meng Delong, Sun Ruochuan, Yun Dapeng, Zhao Zhijie, Lu Daru, Li Yongxiang
Department of General Surgery, First Affiliated Hospital, School of Medicine, Anhui Medical University, Hefei, 230022, Anhui, People's Republic of China.
J Cancer Res Clin Oncol. 2015 Jul;141(7):1265-75. doi: 10.1007/s00432-014-1897-7. Epub 2015 Jan 20.
Eukaryotic elongation factor 1 alpha-2 (eEF1A2) is a protein translation factor involved in protein synthesis. It is overexpressed in various cancers, which indicates potential vital functions in tumorigenesis and progression. Our study aims to investigate the expression levels of eEF1A2 in gastric cancer and its roles in clinical practice.
A total of 129 patients with pathologically confirmed gastric cancer and 24 normal controls were recruited for this study. The expression levels of eEF1A2 in gastric cancer and normal tissues were evaluated by tissue microarrays, quantitative real-time PCR, and western blot analysis. Kaplan-Meier analysis and Cox's proportional hazards model were used in survival analysis.
Compared with corresponding controls, gastric cancer specimens had significantly increased expressions of eEF1A2 at mRNA and protein levels (both P < 0.05). Moreover, multivariate analysis confirmed that overexpression of eEF1A2 was a significant and independent indicator for predicting poor prognosis of gastric cancer.
Our results showed for the first time that overexpression of eEF1A2 was correlated with worse outcomes in gastric cancer patients, suggesting its critical roles in the carcinogenesis of gastric cancer.
真核生物延伸因子1α-2（eEF1A2）是一种参与蛋白质合成的蛋白质翻译因子。它在多种癌症中过表达，这表明其在肿瘤发生和进展中可能具有重要作用。本研究旨在探讨eEF1A2在胃癌中的表达水平及其在临床实践中的作用。
本研究共纳入129例经病理确诊的胃癌患者和24例正常对照。通过组织芯片、定量实时PCR和蛋白质印迹分析评估胃癌组织和正常组织中eEF1A2的表达水平。生存分析采用Kaplan-Meier分析和Cox比例风险模型。
与相应对照相比，胃癌标本中eEF1A2在mRNA和蛋白质水平的表达均显著增加（均P<0.05）。此外，多因素分析证实eEF1A2过表达是预测胃癌预后不良的一个显著且独立的指标。
我们的结果首次表明，eEF1A2过表达与胃癌患者较差的预后相关，提示其在胃癌发生过程中的关键作用。
