Sang Shengbo, Wang Yajun, Feng Qiliang, Wei Ye, Ji Jianlong, Zhang Wendong
a MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering , Taiyuan University of Technology , Taiyuan , Shanxi , China .
b School of Chemistry, Physics and mechanical Engineering , Queensland University of Technology , Brisbane , QLD , Australia , and.
Crit Rev Biotechnol. 2016;36(3):465-81. doi: 10.3109/07388551.2014.991270. Epub 2015 Jan 22.
The detection techniques used in biosensors can be broadly classified into label-based and label-free. Label-based detection relies on the specific properties of labels for detecting a particular target. In contrast, label-free detection is suitable for the target molecules that are not labeled or the screening of analytes which are not easy to tag. Also, more types of label-free biosensors have emerged with developments in biotechnology. The latest developed techniques in label-free biosensors, such as field-effect transistors-based biosensors including carbon nanotube field-effect transistor biosensors, graphene field-effect transistor biosensors and silicon nanowire field-effect transistor biosensors, magnetoelastic biosensors, optical-based biosensors, surface stress-based biosensors and other type of biosensors based on the nanotechnology are discussed. The sensing principles, configurations, sensing performance, applications, advantages and restriction of different label-free based biosensors are considered and discussed in this review. Most concepts included in this survey could certainly be applied to the development of this kind of biosensor in the future.
生物传感器中使用的检测技术可大致分为基于标记的和无标记的。基于标记的检测依赖于标记的特定属性来检测特定目标。相比之下，无标记检测适用于未标记的目标分子或不易标记的分析物的筛选。此外，随着生物技术的发展，出现了更多类型的无标记生物传感器。本文讨论了无标记生物传感器中最新开发的技术，如基于场效应晶体管的生物传感器，包括碳纳米管场效应晶体管生物传感器、石墨烯场效应晶体管生物传感器和硅纳米线场效应晶体管生物传感器、磁弹性生物传感器、基于光学的生物传感器、基于表面应力的生物传感器以及其他基于纳米技术的生物传感器类型。本综述考虑并讨论了不同无标记生物传感器的传感原理、结构、传感性能、应用、优点和局限性。本调查中包含的大多数概念肯定可应用于未来这类生物传感器的开发。
