Aktas Ayse Esin, Yigit Nimet, Aktas Akin, Gozubuyuk Sultan Gamze
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Department of Medical Laboratory, Ataturk University Health Services Vocational School, Erzurum, Turkey.
Eurasian J Med. 2014 Feb;46(1):26-31. doi: 10.5152/eajm.2014.05.
Dermatomycosis is an infection with fungi related to the skin: glabrous skin, hair and/or nails. Oral treatment of fungal infections in dermatology has become a preferred modality for the management of these very common conditions. Although there are increasing numbers of antifungals available for treatment of dermatophytes, some cases and relapses have been unresponsive to treatment. The determination of fungus in-vitro antifungal susceptibility has been reported to be important for the ability to eradicate dermatophytes. It is necessary to perform antifungal susceptibility testing of dermatophytes. E-test (AB Biodisk, Sweden) is a rapid, easy-to-perform in-vitro antifungal susceptibility test. The aim of this study was to investigate the susceptibility of the different species of dermatophyte strains isolated clinical specimens to five antifungal agents using the E-test method.
A total of 66 specimens were collected from the nails, feet, inguinal region, trunk and hands. These strains tested MIC endpoints of E-test for amphotericin B, fluconazole, itraconazole, caspofungin, and ketoconazole were read after 72, and 96 hours incubation for each strain on RPMI 1640 agar.
The dermatophytes tested included Trichophyton rubrum 43 (65.1%), Trichophyton mentagrophytes 7 (10.7%), Microsporum canis 5 (7.6%), Trichophyton tonsurans 5 (7.6%), Epidermophyton floccosum 4 (6.0%) and Trichophyton violaceum 2 (3.0%). The most active agent against all dermatophytes species was caspofungin with a minimal inhibitory concentration (MIC) range (μg/mL(-1)) (0.02-3, 0.032-4, 0.125-0.50, 0.032-2, 0.25-0.50, 0.125-0.50) and it raconazole with an MIC range (μg/mL(-1)) (0.038-1.5, 0.094-1.5, 1-32, 0.016-0.50, 0.25-0.50, 0.125-0.50). The least active agent was fluconazole with an MIC range (μg/mL(-1)) (0, 19-48, 2-256, 2-8, 256, 256, 8-24).
E-test seems to be an alternative method to MIC-determination of antifungal drugs for dermatophytes species, since it is a less-laborious methodology and results could be obtained faster.
皮肤真菌病是由与皮肤相关的真菌引起的感染，包括光滑皮肤、毛发和/或指甲。在皮肤科，真菌感染的口服治疗已成为管理这些常见疾病的首选方式。尽管有越来越多的抗真菌药物可用于治疗皮肤癣菌，但仍有一些病例和复发对治疗无反应。据报道，确定真菌的体外抗真菌药敏性对于根除皮肤癣菌的能力很重要。有必要对皮肤癣菌进行抗真菌药敏试验。E-test（瑞典AB Biodisk公司）是一种快速、易于操作的体外抗真菌药敏试验。本研究的目的是使用E-test方法研究从临床标本中分离出的不同皮肤癣菌菌株对五种抗真菌药物的敏感性。
共收集了66份来自指甲、足部、腹股沟区、躯干和手部的标本。将这些菌株在RPMI 1640琼脂上培养72小时和96小时后，读取两性霉素B、氟康唑、伊曲康唑、卡泊芬净和酮康唑的E-test MIC终点值。
所检测的皮肤癣菌包括红色毛癣菌43株（65.1%）、须癣毛癣菌7株（10.7%）、犬小孢子菌5株（7.6%）、断发毛癣菌5株（7.6%）、絮状表皮癣菌4株（6.0%）和紫色毛癣菌2株（3.0%）。对所有皮肤癣菌种类最有效的药物是卡泊芬净，其最低抑菌浓度（MIC）范围（μg/mL⁻¹）为（0.02 - 3，0.032 - 4，0.125 - 0.50，0.032 - 2，0.25 - 0.50，0.125 - 0.50），伊曲康唑的MIC范围（μg/mL⁻¹）为（0.038 - 1.5，0.094 - 1.5，1 - 32，0.016 - 0.50，0.25 - 0.50，0.125 - 0.50）。最不活跃的药物是氟康唑，其MIC范围（μg/mL⁻¹）为（0，19 - 48，2 - 256，2 - 8，256，256，8 - 24）。
E-test似乎是一种用于测定皮肤癣菌抗真菌药物MIC的替代方法，因为它是一种工作量较小的方法，且能更快获得结果。
