Martínez-Rivera Freddyson J, Natal-Albelo Eduardo J, Martínez Namyr A, Orozco-Vega Roberto A, Muñiz-Seda Oscar A, Barreto-Estrada Jennifer L
Department of Anatomy and Neurobiology, School of Medicine, Medical Sciences Campus, University of Puerto Rico, San Juan, PR 00936, USA.
Department of Biology, Río Piedras Campus, University of Puerto Rico, San Juan, PR 00936, USA.
Behav Processes. 2015 Apr;113:81-5. doi: 10.1016/j.beproc.2015.01.008. Epub 2015 Jan 20.
Adolescents and adults engage in anabolic-androgenic steroid (AAS) misuse seeking their anabolic effects, even though later on, many could develop neuropsychological dependence. Previously, we have shown that nandrolone induces conditioned place preference (CPP) in adult male mice. However, whether nandrolone induces CPP during adolescence remains unknown. In this study, the CPP test was used to determine the rewarding properties of nandrolone (7.5 mg/kg) in adolescent mice. In addition, since D1 dopamine receptors (D1DR) are critical for reward-related processes, the effect of nandrolone on the expression of D1DR in the nucleus accumbens (NAc) was investigated by Western blot analysis. Similar to our previous results, nandrolone induced CPP in adults. However, in adolescents, nandrolone failed to produce place preference. At the molecular level, nandrolone decreased D1DR expression in the NAc only in adult mice. Our data suggest that nandrolone may not be rewarding in adolescents at least during short-term use. The lack of nandrolone rewarding effects in adolescents may be due, in part to differences in D1DR expression during development.
青少年和成年人滥用合成代谢雄激素类固醇（AAS）以寻求其合成代谢作用，尽管后来许多人可能会产生神经心理依赖。此前，我们已经表明，诺龙能在成年雄性小鼠中诱导条件性位置偏爱（CPP）。然而，诺龙在青春期是否会诱导CPP仍不清楚。在本研究中，使用CPP试验来确定诺龙（7.5毫克/千克）对青春期小鼠的奖赏特性。此外，由于D1多巴胺受体（D1DR）对奖赏相关过程至关重要，通过蛋白质免疫印迹分析研究了诺龙对伏隔核（NAc）中D1DR表达的影响。与我们之前的结果相似，诺龙在成年小鼠中诱导了CPP。然而，在青少年中，诺龙未能产生位置偏爱。在分子水平上，诺龙仅在成年小鼠的NAc中降低了D1DR的表达。我们的数据表明，至少在短期使用期间，诺龙可能对青少年没有奖赏作用。青少年缺乏诺龙的奖赏作用可能部分归因于发育过程中D1DR表达的差异。
