Moroz Olga V, Maranta Michelle, Shaghasi Tarana, Harris Paul V, Wilson Keith S, Davies Gideon J
Department of Chemistry, University of York, York Structural Biology Laboratory, York YO10 5DD, England.
Novozymes Inc., 1443 Drew Avenue, Davis, CA 95618, USA.
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):114-20. doi: 10.1107/S2053230X14027307.
The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5 Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.
植物细胞壁纤维素的酶促降解是许多工业过程的核心，包括第二代生物燃料生产。这种解构过程中的关键参与者是真菌纤维二糖水解酶（CBHs），特别是来自碳水化合物活性酶（CAZY）数据库GH7家族的那些酶，通常被称为CBHI酶。本文报道了烟曲霉CBHI Cel7A分别以未结合和结合二糖形式解析的三维结构，分辨率分别为1.8 Å和1.5 Å。在+1和+2亚位点与二糖形成的产物复合物，为深入了解这一与工业相关的生物催化剂家族增加了三维视角。
