Spalthoff S, Jehn P, Zimmerer R, Möllmann U, Gellrich N-C, Kokemueller H
Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.
Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany.
Int J Oral Maxillofac Surg. 2015 Jun;44(6):791-7. doi: 10.1016/j.ijom.2014.11.012. Epub 2015 Jan 22.
We previously generated viable heterotopic bone in living animals and found that 3 months of intrinsic vascularization improved bone formation and matrix degeneration. In this study, we varied the pre-vascularization time to determine its effects on the kinetics of bone formation and ceramic degradation. Two 25-mm-long cylindrical β-tricalcium phosphate scaffolds were filled intraoperatively with autogenous iliac crest bone marrow and implanted in the latissimus dorsi muscle in six sheep. To examine the effect of axial perfusion, one scaffold was surgically implanted with (group C) or without (group D) a central vascular bundle. All animals were sacrificed 6 months postoperatively and histomorphometric measurements were compared to previous results. All implanted scaffolds exhibited ectopic bone growth. However, bone growth was not significantly different between the 3-month (group A, 0.191±0.097 vs. group C, 0.237±0.075; P=0.345) and 6-month (group B, 0.303±0.105 vs. group D, 0.365±0.258; P=0.549) pre-vascularization durations, regardless of vessel supply; early differences between surgically and extrinsically vascularized constructs disappeared after 6 months. Here, we describe a reliable procedure for generating ectopic bone in vivo. A 3-month pre-vascularization duration appears sufficient and ceramic degradation proceeds in accordance with bone generation, supporting the hypothesis of cell-mediated resorption.
我们之前在活体动物中生成了可行的异位骨，并发现3个月的内源性血管化改善了骨形成和基质退变。在本研究中，我们改变了预血管化时间，以确定其对骨形成动力学和陶瓷降解的影响。术中将两根25毫米长的圆柱形β-磷酸三钙支架填充自体髂嵴骨髓，植入6只绵羊的背阔肌中。为了检查轴向灌注的效果，一个支架手术植入（C组）或不植入（D组）中央血管束。所有动物在术后6个月处死，组织形态计量学测量结果与之前的结果进行比较。所有植入的支架均表现出异位骨生长。然而，无论血管供应如何，3个月（A组，0.191±0.097 vs. C组，0.237±0.075；P=0.345）和6个月（B组，0.303±0.105 vs. D组，0.365±0.258；P=0.549）预血管化持续时间的骨生长没有显著差异；手术血管化和外源性血管化构建体之间的早期差异在6个月后消失。在此，我们描述了一种在体内生成异位骨的可靠方法。3个月的预血管化持续时间似乎足够，并且陶瓷降解与骨生成同步进行，支持细胞介导吸收的假设。
