Singh Ravinder, Bollina Venkatesh, Higgins Erin E, Clarke Wayne E, Eynck Christina, Sidebottom Christine, Gugel Richard, Snowdon Rod, Parkin Isobel A P
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada ; School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, 180 009 JK India.
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
Mol Breed. 2015;35(1):35. doi: 10.1007/s11032-015-0224-6. Epub 2015 Jan 21.
, a largely relict crop, has recently returned to interest due to its potential as an industrial oilseed. Molecular markers are key tools that will allow to benefit from modern breeding approaches. Two complementary methodologies, capture of 3' cDNA tags and genomic reduced-representation libraries, both of which exploited second generation sequencing platforms, were used to develop a low density (768) Illumina GoldenGate single nucleotide polymorphism (SNP) array. The array allowed 533 SNP loci to be genetically mapped in a recombinant inbred population of . Alignment of the SNP loci to the genome identified the underlying sequenced regions that would delimit potential candidate genes in any mapping project. In addition, the SNP array was used to assess genetic variation among a collection of 175 accessions of , identifying two sub-populations, yet low overall gene diversity. The SNP loci will provide useful tools for future crop improvement of .
（某种作物），一种基本上处于遗留状态的作物，由于其作为工业油籽的潜力，最近又重新受到关注。分子标记是使（该作物）能够受益于现代育种方法的关键工具。两种互补的方法，即捕获3' cDNA标签和基因组简化代表性文库，这两种方法都利用了第二代测序平台，被用于开发一个低密度（768个）的Illumina GoldenGate单核苷酸多态性（SNP）阵列。该阵列使533个SNP位点能够在（该作物的）一个重组自交群体中进行遗传定位。将SNP位点与（该作物的）基因组进行比对，确定了潜在的测序区域，这些区域将界定任何作图项目中的潜在候选基因。此外，该SNP阵列被用于评估175份（该作物）种质资源中的遗传变异，识别出两个亚群，但总体基因多样性较低。这些SNP位点将为（该作物）未来的作物改良提供有用的工具。
