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利用橄榄渣联合生产生物乙醇:来自摩洛哥中部的木材腐朽真菌作为有前景的分解和发酵生物催化剂

Consolidated bioethanol production from olive mill waste: Wood-decay fungi from central Morocco as promising decomposition and fermentation biocatalysts.

作者信息

Nait M'Barek Hasna, Arif Soukaina, Taidi Behnam, Hajjaj Hassan

机构信息

Faculty of Sciences of Meknes, Laboratory of Plant Biotechnology and Molecular Biology, BP 11201, Zitoune Meknes City, Morocco.

Cluster of Competency «Agri-food, Safety and Security» IUC VLIR-UOS, Moulay Ismail University, Marjane 2, BP 298, Meknes City, Morocco.

出版信息

Biotechnol Rep (Amst). 2020 Oct 9;28:e00541. doi: 10.1016/j.btre.2020.e00541. eCollection 2020 Dec.

DOI:10.1016/j.btre.2020.e00541
PMID:33102160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7578684/
Abstract

Meknes region is a Moroccan olive-processing area generating high amounts of non-valorized Olive Mill Waste (OMW). Fungi are natural decomposers producing varied enzyme classes and effectively contributing to the carbon cycle. However, structural complexity of biomass and modest performances of wild fungi are major limits for local biorefineries. The objective of current research is to assess the ability of local fungi for bioethanol production from OMW using Consolidated Bioprocessing (CBP). This is done by characterizing lignocellulolytic potential of six wood-decay and compost-inhabiting ascomycetes and selecting potent fermentation biocatalysts. High and diversified activities were expressed by and : 9.36 IU. mL and 2.88 IU. mL total cellulase activity, 0.54 IU. mL and 0.57 IU. mL laccase activity, respectively, and 8.43 IU. mL lignin peroxidase activity for the latter. had maximum bioethanol production and yield of 2.47 g.L and 0.84 g.g, respectively, qualifying it as an important bio-agent for single-pot local biorefinery.

摘要

梅克内斯地区是摩洛哥的一个橄榄加工区,会产生大量未得到充分利用的橄榄果渣(OMW)。真菌是天然的分解者,能产生多种酶类,对碳循环有重要贡献。然而,生物质的结构复杂性以及野生真菌的性能一般是当地生物精炼厂面临的主要限制因素。当前研究的目的是评估当地真菌利用联合生物加工(CBP)从OMW生产生物乙醇的能力。这通过表征六种木腐和堆肥中生活的子囊菌的木质纤维素分解潜力并选择高效发酵生物催化剂来实现。 和 表现出较高且多样的活性:总纤维素酶活性分别为9.36 IU·mL和2.88 IU·mL,漆酶活性分别为0.54 IU·mL和0.57 IU·mL,后者的木质素过氧化物酶活性为8.43 IU·mL。 生物乙醇产量和产率最高,分别为2.47 g·L和0.84 g·g,使其成为单罐式当地生物精炼厂的重要生物制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/a51e9863cc49/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/248cdef99035/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/2d1f38c97775/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/e4188ab6bc27/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/17ce74aafb88/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/a51e9863cc49/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/248cdef99035/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/2d1f38c97775/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/e4188ab6bc27/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/17ce74aafb88/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8713/7578684/a51e9863cc49/gr4.jpg

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