Harada J J, Barker S J, Goldberg R B
Department of Biology, University of California, Los Angeles 90024-1606.
Plant Cell. 1989 Apr;1(4):415-25. doi: 10.1105/tpc.1.4.415.
We investigated the chromosomal organization and developmental regulation of soybean beta-conglycinin genes. The beta-conglycinin gene family contains at least 15 members divided into two major groups encoding 2.5-kilobase and 1.7-kilobase embryo mRNAs. beta-Conglycinin genes are clustered in several DNA regions and are highly homologous along their entire lengths. The two groups differ by the presence or absence of specific DNA segments. These DNA segments account for the size differences in beta-conglycinin mRNAs. The 2.5-kilobase and 1.7-kilobase beta-conglycinin mRNAs accumulate and decay at different times during embryogenesis. By contrast, genes encoding these mRNAs are transcriptionally activated and repressed at the same time periods. Our studies indicate that the beta-conglycinin family evolved by both duplication and insertion/deletion events, and that beta-conglycinin gene expression is regulated at both the transcriptional and posttranscriptional levels.
我们研究了大豆β-伴大豆球蛋白基因的染色体组织和发育调控。β-伴大豆球蛋白基因家族至少包含15个成员，分为两个主要组，分别编码2.5千碱基和1.7千碱基的胚胎mRNA。β-伴大豆球蛋白基因聚集在几个DNA区域，并且在其全长上具有高度同源性。这两组的区别在于特定DNA片段的有无。这些DNA片段导致了β-伴大豆球蛋白mRNA的大小差异。2.5千碱基和1.7千碱基的β-伴大豆球蛋白mRNA在胚胎发生过程中的不同时间积累和降解。相比之下，编码这些mRNA的基因在相同的时间段被转录激活和抑制。我们的研究表明，β-伴大豆球蛋白家族通过复制和插入/缺失事件进化而来，并且β-伴大豆球蛋白基因的表达在转录和转录后水平上均受到调控。
