艰难梭菌实验室菌株630Δerm的全基因组序列揭示了其与菌株630的差异，包括移动元件CTn5的易位。

Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5.

作者信息

van Eijk Erika, Anvar Seyed Yahya, Browne Hilary P, Leung Wai Yi, Frank Jeroen, Schmitz Arnoud M, Roberts Adam P, Smits Wiep Klaas

机构信息

Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands.

Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.

出版信息

BMC Genomics. 2015 Jan 31;16(1):31. doi: 10.1186/s12864-015-1252-7.

BACKGROUND

Clostridium difficile strain 630Δerm is a spontaneous erythromycin sensitive derivative of the reference strain 630 obtained by serial passaging in antibiotic-free media. It is widely used as a defined and tractable C. difficile strain. Though largely similar to the ancestral strain, it demonstrates phenotypic differences that might be the result of underlying genetic changes. Here, we performed a de novo assembly based on single-molecule real-time sequencing and an analysis of major methylation patterns.

RESULTS

In addition to single nucleotide polymorphisms and various indels, we found that the mobile element CTn5 is present in the gene encoding the methyltransferase rumA rather than adhesin CD1844 where it is located in the reference strain.

CONCLUSIONS

Together, the genetic features identified in this study may help to explain at least part of the phenotypic differences. The annotated genome sequence of this lab strain, including the first analysis of major methylation patterns, will be a valuable resource for genetic research on C. difficile.