Ilic-Tomic Tatjana, Genčić Marija S, Živković Milena Z, Vasiljevic Branka, Djokic Lidija, Nikodinovic-Runic Jasmina, Radulović Niko S
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, Belgrade, 11010, Serbia.
Appl Microbiol Biotechnol. 2015 Jun;99(11):4815-33. doi: 10.1007/s00253-014-6364-5. Epub 2015 Jan 31.
Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 °C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C7-C30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
本文描述了一种新型土壤细菌链霉菌属NP10，它能够在不同于链霉菌通常最适生长条件的环境下生长（例如，4℃、pH 9以及高氯化钠浓度），具有非典型的溶血、DNA酶和纤维素分解活性。该菌株产生并分泌大量游离长链脂肪酸（FAs）到生长培养基中。一项同步进行的脂质组学研究揭示了该菌株产生的脂肪酸具有很大的结构多样性，有超过50种不同的正构和支链、饱和及环丙烷脂肪酸（C7 - C30）。其中两种，i - 17:0cy9 - 10和a - 18:0cy9 - 10，代表新的天然产物，也是首次鉴定出的支链环丙烷脂肪酸。链霉菌属NP10的游离脂质和结合脂质谱均以饱和支链脂肪酸（i - 14:0、a - 15:0和i - 16:0）为主。尽管这些游离脂肪酸仅表现出中等抗菌活性，但我们的结果表明它们可能在与另一种土壤微生物铜绿假单胞菌的种间信号传递中具有生态生理作用。这项工作是关于链霉菌中游离脂肪酸库结构多样性和复杂性的首份全面报告。一种天然存在的链霉菌，如链霉菌属NP10，能向培养基中分泌大量游离长链脂肪酸（无细胞毒性），这可能在微生物生物柴油生产中有用。
