Cerio R, Spaull J, Jones E W
Institute of Dermatology, United Medical School of Guy's Hospital, London, U.K.
Br J Dermatol. 1989 Feb;120(2):197-206. doi: 10.1111/j.1365-2133.1989.tb07783.x.
The histogenesis of histiocytoma (dermatofibroma) was investigated using new antibodies that demonstrate factor XIIIa (FXIIIa) positive cells and the monocyte macrophage cell series (MAC 387), in formalin fixed tissue. The distribution of S100 protein, vimentin and Ulex europaeus agglutinin I (UEA-I) were also studied. The antibody against FXIIIa labelled the normal dermal population of fixed connective tissue cells (dermal dendrocytes) emphasizing their dendritic processes; cells that are widely distributed, but are most numerous in the papillary dermis. In contrast, the antibody, MAC 387 against monocyte derived macrophages, did not label this cell population. In 30 histiocytomas, intense labelling for FXIIIa was found peripherally, but labelling was rather weaker in cells situated centrally. Only a few cells labelled for S100 protein and with the monoclonal antibody MAC 387, but vimentin positivity was universal. UEA-I labelled only vessels in histiocytomas. However, FXIIIa labelling was negative in 16 dermatofibrosarcomas, three keloids and several other fibroproliferative lesions. These contrasting results with FXIIIa labelling have practical implications for diagnosis of benign and malignant spindle cell tumours of the skin. We conclude that histiocytomas (dermal dendrocytomas) take their origin from dermal fixed connective tissue cells, (dermal dendrocytes), but other spindle cell tumours may differ in histogenesis.
利用新型抗体对组织细胞瘤（皮肤纤维瘤）的组织发生进行了研究，这些抗体可在福尔马林固定组织中显示因子ⅩⅢa（FXⅢa）阳性细胞和单核巨噬细胞系列（MAC 387）。还研究了S100蛋白、波形蛋白和欧洲荆豆凝集素I（UEA-I）的分布。抗FXⅢa抗体标记了固定结缔组织细胞（真皮树突状细胞）的正常真皮群体，突出了它们的树突状突起；这些细胞分布广泛，但在乳头层真皮中数量最多。相比之下，抗单核细胞衍生巨噬细胞的抗体MAC 387未标记该细胞群体。在30例组织细胞瘤中，发现外周FXⅢa标记强烈，但中央部位的细胞标记较弱。只有少数细胞被S100蛋白和单克隆抗体MAC 387标记，但波形蛋白阳性普遍存在。UEA-I仅标记组织细胞瘤中的血管。然而，在16例皮肤纤维肉瘤、3例瘢痕疙瘩和其他几种纤维增生性病变中，FXⅢa标记为阴性。FXⅢa标记的这些对比结果对皮肤良恶性梭形细胞瘤的诊断具有实际意义。我们得出结论，组织细胞瘤（真皮树突状细胞瘤）起源于真皮固定结缔组织细胞（真皮树突状细胞），但其他梭形细胞瘤在组织发生上可能有所不同。
