Surman František, Riedel Tomáš, Bruns Michael, Kostina Nina Yu, Sedláková Zdeňka, Rodriguez-Emmenegger Cesar
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Prague, 162 06, Czech Republic.
Institute for Applied Materials (IAM), Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Macromol Biosci. 2015 May;15(5):636-46. doi: 10.1002/mabi.201400470. Epub 2015 Jan 21.
In the current study, well-defined polymer brushes are shown as an effective surface modification to resist the adhesion of whole blood and its components. Poly[oligo(ethylene glycol)methylether methacrylate] (poly(MeOEGMA)), poly(hydroxyethyl methacrylate) (poly(HEMA)), poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)), and poly(carboxybetaine acrylamide) (poly(CBAA)) brushes were grown by surface initiated atom transfer radical polymerization (SI-ATRP) and subsequently characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), dynamic contact angle measurements, atomic force microscopy (AFM), and surface plasmon resonance (SPR) spectroscopy. All brushes decreased the fouling from blood plasma over 95% and prevented the adhesion of platelets, erythrocytes, and leukocytes as evidenced by SPR and SEM measurements.
在当前研究中，定义明确的聚合物刷被证明是一种有效的表面改性方法，可抵抗全血及其成分的粘附。通过表面引发原子转移自由基聚合（SI-ATRP）制备了聚[寡聚（乙二醇）甲基醚甲基丙烯酸酯]（聚（MeOEGMA））、聚（甲基丙烯酸羟乙酯）（聚（HEMA））、聚[N-（2-羟丙基）甲基丙烯酰胺]（聚（HPMA））和聚（羧基甜菜碱丙烯酰胺）（聚（CBAA））刷，随后通过傅里叶变换红外（FTIR）光谱、X射线光电子能谱（XPS）、动态接触角测量、原子力显微镜（AFM）和表面等离子体共振（SPR）光谱对其进行了表征。如SPR和SEM测量所示，所有刷均使血浆污垢减少超过95%，并防止了血小板、红细胞和白细胞的粘附。
