Liu Rongfang, Fang Xianhai, Meng Chao, Xing Jingchun, Liu Jinfeng, Yang Wanchao, Li Wenzhi, Zhou Huacheng
Department of Anesthesiology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China Hei Long Jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150081, China.
Department of Anesthesiology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China Hei Long Jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin 150081, China
Exp Biol Med (Maywood). 2015 Sep;240(9):1214-22. doi: 10.1177/1535370214563895. Epub 2015 Feb 7.
Hydrogen has antioxidant and anti-inflammatory effects on lung ischemia-reperfusion injury when it is inhaled by donor or/and recipient. This study examined the effects of lung inflation with 3% hydrogen during the cold ischemia phase on lung graft function in rats. The donor lung was inflated with 3% hydrogen, 40% oxygen, and 57% nitrogen at 5 mL/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. In the control group, the donor lung was inflated with 40% oxygen and 60% nitrogen at 5 mL/kg. The recipient was euthanized 2 h after orthotropic lung transplantation. The hydrogen concentration in the donor lung during the cold ischemia phase was 1.99-3%. The oxygenation indices in the arterial blood and pulmonary vein blood were improved in the hydrogen group. The inflammation response indices, including lung W/D ratio, the myeloperoxidase activity in the grafts, and the levels of IL-8 and TNF-α in serum, were significantly lower in the hydrogen group (5.2 ± 0.8, 0.76 ± 0.32 U/g, 340 ± 84 pg/mL, and 405 ± 115 pg/mL, respectively) than those in the control group (6.5 ± 0.7, 1.1 ± 0.5 U/g, 443 ± 94 pg/mL, and 657 ± 96 pg/mL, respectively (P < 0.05), and the oxidative stress indices, including the superoxide dismutase activity and the level of malonaldehyde in lung grafts were improved after hydrogen application. Furthermore, the lung injury score determined by histopathology, the cell apoptotic index, and the caspase-3 protein expression in lung grafts were decreased after hydrogen treatment, and the static pressure-volume curve of lung graft was improved by hydrogen inflation. In conclusion, lung inflation with 3% hydrogen during the cold ischemia phase alleviated lung graft injury and improved graft function.
当供体或/和受体吸入氢气时，氢气对肺缺血再灌注损伤具有抗氧化和抗炎作用。本研究检测了在冷缺血期用3%氢气对大鼠肺移植功能进行肺充气的效果。供体肺以5 mL/kg的剂量用3%氢气、40%氧气和57%氮气进行充气，在冷缺血期每20分钟更换一次气体，持续2小时。在对照组中，供体肺以5 mL/kg的剂量用40%氧气和60%氮气进行充气。在原位肺移植术后2小时对受体实施安乐死。冷缺血期供体肺中的氢气浓度为1.99% - 3%。氢气组动脉血和肺静脉血中的氧合指数得到改善。氢气组的炎症反应指标，包括肺湿干重比、移植物中的髓过氧化物酶活性以及血清中IL - 8和TNF -α的水平，分别显著低于对照组（分别为5.2±0.8、0.76±0.32 U/g、340±84 pg/mL和405±115 pg/mL）（分别为6.5±0.7、1.1±0.5 U/g、443±94 pg/mL和657±96 pg/mL）（P < 0.05），并且应用氢气后肺移植物中的氧化应激指标，包括超氧化物歧化酶活性和丙二醛水平得到改善。此外，氢气处理后通过组织病理学确定的肺损伤评分、细胞凋亡指数以及肺移植物中caspase - 3蛋白表达均降低，并且氢气充气改善了肺移植物的静态压力 - 容积曲线。总之，在冷缺血期用3%氢气进行肺充气可减轻肺移植物损伤并改善移植物功能。
