从植物材料中分离出的新物种——柯氏嗜干菌的描述及图尔库嗜干菌的修订描述
Description of Siccibacter colletis sp. nov., a novel species isolated from plant material, and emended description of Siccibacter turicensis.
作者信息
Jackson Emily E, Masood Naqash, Ibrahim Khaled, Urvoy Noémie, Hariri Sumyya, Forsythe Stephen J
机构信息
School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Université Paris Est Créteil, Département Génie Biologique, 61 Avenue du Général de Gaulle, 94000 Creteil, France.
出版信息
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1335-1341. doi: 10.1099/ijs.0.000108. Epub 2015 Feb 9.
A re-evaluation of the taxonomic position of two strains, 1383(T) and 2249, isolated from poppy seeds and tea leaves, which had been identified as Siccibacter turicensis (formerly Cronobacter zurichensis ), was carried out. The analysis included phenotypic characterization, 16S rRNA gene sequencing, multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, fusA, glnS, gyrB and infB; 2034 bp) and ribosomal MLSA (53 loci; 22 511 bp). 16S rRNA gene sequence analysis and MLSA showed that the strains formed an independent phylogenetic lineage, with Siccibacter turicensis LMG 23730(T) as the closest neighbour. Average nucleotide identity analysis and phenotypic analysis confirmed that these strains represent a novel species, for which the name Siccibacter colletis sp. nov. is proposed. The type strain is 1383(T) ( = NCTC 14934(T) = CECT 8567(T) = LMG 28204(T)). An emended description of Siccibacter turicensis is also provided.
对从罂粟种子和茶叶中分离出的两株菌株1383(T)和2249的分类地位进行了重新评估，这两株菌株之前被鉴定为图里西芽孢杆菌（以前称为苏黎世克罗诺杆菌）。分析包括表型特征分析、16S rRNA基因测序、五个管家基因（atpD、fusA、glnS、gyrB和infB；2034 bp）的多位点序列分析（MLSA）以及核糖体MLSA（53个位点；22511 bp）。16S rRNA基因序列分析和MLSA表明，这些菌株形成了一个独立的系统发育谱系，与图里西芽孢杆菌LMG 23730(T)亲缘关系最近。平均核苷酸同一性分析和表型分析证实，这些菌株代表一个新物种，为此提议将其命名为科氏芽孢杆菌（Siccibacter colletis sp. nov.）。模式菌株为1383(T)（=NCTC 14934(T)=CECT 8567(T)=LMG 28204(T)）。还提供了图里西芽孢杆菌的修订描述。
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