利用种间杂交将Abll定位在与人类1号染色体同线的小鼠1号染色体远端保守连锁群内。

Mapping of Abll within a conserved linkage group on distal mouse chromosome 1 syntenic with human chromosome 1 using an interspecific cross.

作者信息

Seldin M F, Kruh G D

机构信息

Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.

出版信息

Genomics. 1989 Feb;4(2):221-3. doi: 10.1016/0888-7543(89)90305-4.

DOI:10.1016/0888-7543(89)90305-4

PMID:2567701

Abstract

A human Abelson related gene (ABLL) cDNA clone was used to detect restriction fragment length polymorphisms (RFLPs) on mouse Southern blots. Abll was mapped to mouse chromosome 1 by analysis of segregation with other distal chromosome 1 genetic polymorphisms by using a panel of DNAs from [(C3H/HeJ-gld/gld x Mus spretus) F1 x C3H/HeJ-gld/gld] interspecific backcross mice. The data indicate the following gene order: (centromere)-CD45-6.5 cM-Lamb-2-1 cM-Abll-2 cM-At-3. The results extend the analysis of a large conserved linkage group spanning nearly 30 cM on distal mouse chromosome 1 syntenic with human chromosome 1q21-32. Within this linkage group similar relative positions have been characterized in both species for C4BP, REN, CD45, LAMB2, ABLL, AT3, APOA2, and SPTA.

摘要

使用人类阿贝尔森相关基因（ABLL）cDNA克隆来检测小鼠Southern印迹上的限制性片段长度多态性（RFLP）。通过使用来自[（C3H/HeJ-gld/gld×小家鼠）F1×C3H/HeJ-gld/gld]种间回交小鼠的一组DNA，通过与其他1号染色体远端遗传多态性的分离分析，将Abll定位到小鼠1号染色体上。数据表明以下基因顺序：（着丝粒）-CD45-6.5厘摩-Lamb-2-1厘摩-Abll-2厘摩-At-3。这些结果扩展了对一个大型保守连锁群的分析，该连锁群跨越小鼠1号染色体远端近30厘摩，与人1号染色体q21-32同线。在这个连锁群中，C4BP、REN、CD45、LAMB2、ABLL、AT3、APOA2和SPTA在两个物种中的相对位置相似。