小鼠1号染色体远端分子遗传图谱的建立：与人类1号染色体长臂同源的保守连锁群的进一步界定。

Establishment of a molecular genetic map of distal mouse chromosome 1: further definition of a conserved linkage group syntenic with human chromosome 1q.

作者信息

Seldin M F, Morse H C, LeBoeuf R C, Steinberg A D

机构信息

Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.

出版信息

Genomics. 1988 Jan;2(1):48-56. doi: 10.1016/0888-7543(88)90108-5.

DOI:10.1016/0888-7543(88)90108-5

PMID:3384439

Abstract

A linkage map of distal mouse chromosome 1 was constructed by restriction fragment length polymorphism analysis of DNAs from seven sets of recombinant inbred (RI) strains. The data obtained with seven probes on Southern hybridization combined with data from previous studies suggest the gene order Cfh, Pep-3/Ren-1,2, Ly-5, Lamb-2, At-3, Apoa-2/Ly-17,Spna-1. These results confirm and extend analyses of a large linkage group which includes genes present on a 20-30 cM span of mouse chromosome 1 and those localized to human chromosome 1q21-32. Moreover, the data indicate similar relative positions of human and mouse complement receptor-related genes REN, CD45, LAMB2, AT3, APOA2, and SPTA. These results suggest that mouse gene analyses may help in detailed mapping of human genes within such a syntenic group.

摘要

通过对七组重组近交（RI）品系的DNA进行限制性片段长度多态性分析，构建了小鼠1号染色体远端的连锁图谱。用七个探针进行Southern杂交获得的数据，结合先前研究的数据，表明基因顺序为Cfh、Pep-3/Ren-1,2、Ly-5、Lamb-2、At-3、Apoa-2/Ly-17、Spna-1。这些结果证实并扩展了对一个大型连锁群的分析，该连锁群包括存在于小鼠1号染色体20-30 cM跨度上的基因以及定位到人类1号染色体1q21-32的基因。此外，数据表明人类和小鼠补体受体相关基因REN、CD45、LAMB2、AT3、APOA2和SPTA的相对位置相似。这些结果表明，小鼠基因分析可能有助于在此类同线群内对人类基因进行详细定位。