Azizi Hamidreza, Shiran Behrouz, Boroujeni Arash Borjian, Jafari Milad
Department of Pathobiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Department of Biotechnology and Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
Iran J Parasitol. 2014 Sep;9(3):429-34.
Toxoplasmosis is a worldwide spread disease. The present study examined the prevalence of Toxoplasma gondii infection among animals of edible meat (cattle and sheep) in Chaharmahal va Bakhtiari Province (Southwest of Iran) in 2012. Furthermore, we attempted for the first time to identify this parasite from the meat products in the province.
The tongue, brain, femur muscle and liver of 50 sheep and 70 cattle as well as 50 samples of meat products were selected and collected to perform molecular survey using Nested-PCR method.
Of the studied sheep, 38% were infected. The infection rate in the age groups under 1 year, 1-2 years, and more than 2 years was 25%, 35.29% and 52.94%, respectively. The infection rate in femur muscle, brain, liver and tongue was 28%, 32%, 30% and 16%, respectively. Of the studied cattle, 8.57% were infected. The infection rate in the age groups 1-2 years, 2-4 years, and more than 4 years was 3.7%, 9.09% and 14.28%, respectively. Sheep was infected 6 times more than cattle (OR = 6.53 CI = 2.374-18.005).The infection rate among samples of meat products was 12% (6 samples out of 50 samples).
Due to the high rate of this parasitic infection among the slaughtered animals as well as meat products in this region, the use of infected material can be one of the main risk factors of transmission of the parasite to humans.
弓形虫病是一种在全球范围内传播的疾病。本研究调查了2012年伊朗西南部恰哈马哈勒-巴赫蒂亚里省可食用肉类动物（牛和羊）中弓形虫感染的流行情况。此外，我们首次尝试从该省的肉类产品中鉴定这种寄生虫。
选取并收集50只绵羊、70头牛的舌头、大脑、股骨肌肉和肝脏以及50份肉类产品样本，采用巢式聚合酶链反应方法进行分子检测。
在所研究的绵羊中，38%受到感染。1岁以下、1 - 2岁和2岁以上年龄组的感染率分别为25%、35.29%和52.94%。股骨肌肉、大脑、肝脏和舌头的感染率分别为28%、32%、30%和16%。在所研究的牛中，8.57%受到感染。1 - 2岁、2 - 4岁和4岁以上年龄组的感染率分别为3.7%、9.09%和14.28%。绵羊的感染率是牛的6倍（比值比=6.53，可信区间=2.374 - 18.005）。肉类产品样本中的感染率为12%（50个样本中有6个样本）。
由于该地区屠宰动物及肉类产品中这种寄生虫感染率较高，使用受感染的材料可能是该寄生虫传播给人类的主要风险因素之一。
