Couto Diana, Sousa Rosa, Andrade Lara, Leander Magdalena, Lopez-Quintela M Arturo, Rivas José, Freitas Paulo, Lima Margarida, Porto Graça, Porto Beatriz, Carvalho Félix, Fernandes Eduarda
UCIBIO/REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
Laboratory of Cytogenetics, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Toxicol Lett. 2015 Apr 16;234(2):67-73. doi: 10.1016/j.toxlet.2015.02.010. Epub 2015 Feb 12.
The use of iron oxide nanoparticles (ION) for diagnostic and therapeutic purposes requires a clear favorable risk-benefit ratio. This work was performed with the aim of studying the ability of polyacrylic acid (PAA)-coated and non-coated ION to induce genotoxicity in human T lymphocytes. For that purpose, their influence on cell cycle progression and on the induction of chromosome aberrations was evaluated. Blood samples collected from healthy human donors were exposed to PAA-coated and non-coated ION, at different concentrations, for 48h. The obtained results showed that, for all culture conditions, the tested ION are not genotoxic and do not influence the cell cycle arrest. Their possible cumulative effect with the iron-dependent genotoxic agent BLM was also evaluated. Blood samples collected from healthy human donors were exposed to ION, at different concentrations, for 48h, in the presence of a pre-determined toxic concentration of BLM. The obtained results showed that, for all culture conditions, the tested ION do not potentiate the clastogenic effects of BLM.
将氧化铁纳米颗粒（ION）用于诊断和治疗目的需要明确的有利风险效益比。这项工作旨在研究聚丙烯酸（PAA）包被和未包被的ION诱导人T淋巴细胞遗传毒性的能力。为此，评估了它们对细胞周期进程和染色体畸变诱导的影响。从健康人类供体采集的血样在不同浓度下分别暴露于PAA包被和未包被的ION中48小时。所得结果表明，在所有培养条件下，所测试的ION均无遗传毒性且不影响细胞周期停滞。还评估了它们与铁依赖性遗传毒性剂博来霉素（BLM）可能的累积效应。从健康人类供体采集的血样在存在预先确定的有毒浓度BLM的情况下，在不同浓度下暴露于ION 48小时。所得结果表明，在所有培养条件下，所测试的ION均不会增强BLM的致断裂效应。
