Palomino-Schätzlein Martina, García Hermenegildo, Gutiérrez-Carcedo Patricia, Pineda-Lucena Antonio, Herance José Raul
Laboratorio de Bioquímica Estructural, Centro de Investigación Príncipe Felipe, Valencia, Spain.
Instituto Universitario de Tecnología Química CSIC-UPV, Valencia, Spain.
PLoS One. 2017 Aug 9;12(8):e0182985. doi: 10.1371/journal.pone.0182985. eCollection 2017.
Human peripheral blood cells are relevant ex vivo models for characterizing diseases and evaluating the pharmacological effects of therapeutic interventions, as they provide a close reflection of an individual pathophysiological state. In this work, a new approach to evaluate the impact of nanoparticles on the three main fractions of human peripheral blood cells by nuclear magnetic resonance spectroscopy is shown. Thus, a comprehensive protocol has been set-up including the separation of blood cells, their in vitro treatment with nanoparticles and the extraction and characterization of metabolites by nuclear magnetic resonance. This method was applied to assess the effect of gold nanoparticles, either coated with chitosan or supported on ceria, on peripheral blood cells from healthy individuals. A clear antioxidant effect was observed for chitosan-coated gold nanoparticles by a significant increase in reduced glutathione, that was much less pronounced for gold-cerium nanoparticles. In addition, the analysis revealed significant alterations of several other pathways, which were stronger for gold-cerium nanoparticles. These results are in accordance with the toxicological data previously reported for these materials, confirming the value of the current methodology.
人类外周血细胞是用于疾病特征描述和评估治疗干预措施药理作用的相关离体模型，因为它们能密切反映个体的病理生理状态。在这项工作中，展示了一种通过核磁共振光谱评估纳米颗粒对人类外周血细胞三个主要部分影响的新方法。因此，已建立了一个全面的方案，包括血细胞的分离、用纳米颗粒进行体外处理以及通过核磁共振对代谢物进行提取和表征。该方法用于评估壳聚糖包被或负载于二氧化铈上的金纳米颗粒对健康个体外周血细胞的影响。观察到壳聚糖包被的金纳米颗粒具有明显的抗氧化作用，还原型谷胱甘肽显著增加，而金 - 铈纳米颗粒的这种作用则不太明显。此外，分析还揭示了其他几个途径的显著变化，金 - 铈纳米颗粒的这些变化更为强烈。这些结果与先前报道的这些材料的毒理学数据一致，证实了当前方法的价值。
