Kiss Balázs, László János F, Szalai Andrea, Pórszász Róbert
Department of Biophysics and Radiation Biology and MTA-SE Molecular Biophysics Research Group, Semmelweis University, Budapest, Hungary.
Department of Computer Science, University of Debrecen, Debrecen, Hungary; e-Comers LLC, Budapest, Hungary.
PLoS One. 2015 Feb 19;10(2):e0118089. doi: 10.1371/journal.pone.0118089. eCollection 2015.
The effect static magnetic field (SMF)-exposure may exert on edema development has been investigated. A 6 h long whole-body (WBSMF) or local (LSMF), continuous, inhomogeneous SMF-exposure was applied on anesthetized mice in an in vivo model of mustard oil (MO)-induced ear edema. LSMF was applied below the treated ear, below the lumbar spine, or below the mandible. Ear thickness (v) was checked 8 times during the exposure period (at 0, 0.25, 1, 2, 3, 4, 5, and 6 h). The effect size of the applied treatment (η) on ear thickness was calculated by the formula η = 100% × (1-v(j)/v(i)), where group i is the control group and j is the treated group. Results showed that MO treatment in itself induced a significant ear edema with an effect of 9% (p<0.001). WBSMF or LSMF on the spine in combination with MO treatment increased ear thickness even further resulting in an effect of η>11% in both cases compared to SMF-exposure alone (p<0.001). In these cases SMF-exposure alone without MO treatment reduced ear thickness significantly (p<0.05), but within estimated experimental error. In cases of LSMF-exposure on the head, a significant SMF-exposure induced ear thickness reduction was found (η = 5%, p<0.05). LSMF-exposure on the spine affected ear thickness with and without MO treatment almost identically, which provides evidence that the place of local SMF action may be in the lower spinal region.
研究了静磁场（SMF）暴露对水肿发展可能产生的影响。在芥子油（MO）诱导的耳水肿体内模型中，对麻醉小鼠进行了6小时的全身（WBSMF）或局部（LSMF）、连续、不均匀的SMF暴露。LSMF施加于治疗耳下方、腰椎下方或下颌下方。在暴露期间（0、0.25、1、2、3、4、5和6小时）检查8次耳厚度（v）。应用治疗（η）对耳厚度的效应大小通过公式η = 100% × (1 - v(j)/v(i))计算，其中i组为对照组，j组为治疗组。结果表明，MO治疗本身可诱导显著的耳水肿，效应为9%（p<0.001）。与单独的SMF暴露相比，脊柱上的WBSMF或LSMF与MO治疗联合使用进一步增加了耳厚度，两种情况下的效应均为η>11%（p<0.001）。在这些情况下，单独的SMF暴露而无MO治疗可显著降低耳厚度（p<0.05），但在估计的实验误差范围内。在头部进行LSMF暴露的情况下，发现SMF暴露可显著降低耳厚度（η = 5%，p<0.05）。无论有无MO治疗，脊柱上的LSMF暴露对耳厚度的影响几乎相同，这提供了局部SMF作用部位可能在脊髓下部区域的证据。
