Sousa-Martins Diogo, Caseli Luciano, Figueiredo Mafalda C, Sa E Cunha Claudia, Mota-Filipe Helder, Souza-Lima Acácio, Belfort Rubens, Rodrigues Eduardo, Maia Mauricio
Department of Social Pharmacy, College of Pharmacy, University of Lisbon, Lisbon, Portugal Department of Ophthalmology, Escola Paulista de Medicina, Federal University of São Paulo (Unifesp), São Paulo, Brazil.
Department of Earth and Exacts Sciences, Federal University of São Paulo (Unifesp), Diadema, São Paulo, Brazil.
Invest Ophthalmol Vis Sci. 2015 Feb 19;56(3):1993-2000. doi: 10.1167/iovs.14-16187.
To investigate and compare the mechanism by which lutein-based and synthetic intraocular dyes interact with their target membranes during ophthalmic surgeries.
Surrogate membrane models were used in order to simulate the different intraocular membranes: internal limiting membrane (ILM), vitreous, anterior capsule (AC), and epiretinal membrane (ERM). Different lutein-based dyes, such as Phacodyne, Retidyne, Retidyne Plus, and Vitreodyne were tested, as well as Trypan Blue (TB), Indocyanine Green (ICG), Brilliant Blue (BB), and Triamcinolone Acetonide (TA). The interactions between the film components occurring at the air-water interface were investigated with surface pressure-area isotherms and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS).
With the exception of TA and ICG, none of the tested dyes revealed toxicity to the analyzed membranes. The interaction of TA with the vitreous model affected deeply the biointerface structure of the model. A significant condensation of the monolayer is noted when ICG contacted with ILM by the isotherms or even a solubilization of part of the monolayer toward the aqueous subphase. Retidyne Plus may provide the fluidization of the membrane, but maintains intact the structure of proteins present in the model.
The present study demonstrates for the first time that lutein-based dyes interact through a physical mechanism of action with membrane models of structures present in human eye. On the other hand, the chemical interaction of synthetic dyes TA and ICG resulted in an alteration of the membrane models.
研究并比较基于叶黄素的眼内染料和合成眼内染料在眼科手术过程中与目标膜相互作用的机制。
使用替代膜模型来模拟不同的眼内膜：内界膜（ILM）、玻璃体、前囊膜（AC）和视网膜前膜（ERM）。测试了不同的基于叶黄素的染料，如Phacodyne、Retidyne、Retidyne Plus和Vitreodyne，以及台盼蓝（TB）、吲哚菁绿（ICG）、亮蓝（BB）和曲安奈德（TA）。利用表面压力-面积等温线和偏振调制红外反射吸收光谱（PM-IRRAS）研究了在气-水界面发生的膜成分之间的相互作用。
除TA和ICG外，所测试的染料均未对分析的膜显示出毒性。TA与玻璃体模型的相互作用对模型的生物界面结构有深远影响。当ICG通过等温线与ILM接触时，单层膜会发生显著凝聚，甚至部分单层膜会向水相溶解。Retidyne Plus可能使膜流化，但能保持模型中存在的蛋白质结构完整。
本研究首次证明基于叶黄素的染料通过物理作用机制与人眼内存在的结构的膜模型相互作用。另一方面，合成染料TA和ICG的化学相互作用导致膜模型发生改变。
