Serrano-Fujarte Isela, López-Romero Everardo, Reyna-López Georgina Elena, Martínez-Gámez Ma Alejandrina, Vega-González Arturo, Cuéllar-Cruz Mayra
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Campus Guanajuato, Noria Alta S/N, 36050 Guanajuato, GTO, Mexico.
Centro de Investigaciones en Optica AC, Loma del Bosque 115, Col. Lomas del Campestre, 37150 León, GTO, Mexico.
Biomed Res Int. 2015;2015:783639. doi: 10.1155/2015/783639. Epub 2015 Feb 1.
The aims of the study were to evaluate the influence of culture media on biofilm formation by C. albicans, C. glabrata, C. krusei, and C. parapsilosis and to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, the Candida species were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H2O2, menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay. C. albicans formed biofilms preferentially in YPD containing 2% glucose (YPD/2%), C. glabrata in glucose-free YNB or supplemented with 0.2% glucose (YNB/0.2%), while C. krusei and C. parapsilosis preferred YP, YPD/0.2%, and YPD/2%. Interestingly, only C. albicans produced an exopolymeric matrix. This is the first report dealing with the in vitro effect of the culture medium and glucose on the formation of biofilms in four Candida species as well as the resistance of sessile cells to antifungals, AgNPs, and ROS. Our results suggest that candidiasis in vivo is a multifactorial and complex process where the nutritional conditions, the human immune system, and the adaptability of the pathogen should be considered altogether to provide an effective treatment of the patient.
本研究的目的是评估培养基对白色念珠菌、光滑念珠菌、克柔念珠菌和近平滑念珠菌生物膜形成的影响，并研究与浮游细胞相比，固着细胞对抗真菌药物和活性氧（ROS）的反应。为了形成生物膜，念珠菌在补充或不补充0.2%或2%葡萄糖的YP或YNB培养基中培养不同时间。将固着细胞和浮游细胞暴露于浓度不断增加的抗真菌药物、过氧化氢、甲萘醌或银纳米颗粒（AgNP）中。通过扫描电子显微镜（SEM）观察生物膜，并通过XTT法进行定量。白色念珠菌优先在含2%葡萄糖的YPD（YPD/2%）中形成生物膜，光滑念珠菌在无葡萄糖的YNB或补充0.2%葡萄糖的YNB（YNB/0.2%）中形成生物膜，而克柔念珠菌和近平滑念珠菌则更喜欢YP、YPD/0.2%和YPD/2%。有趣的是，只有白色念珠菌产生胞外基质。这是第一份关于培养基和葡萄糖对四种念珠菌生物膜形成的体外影响以及固着细胞对抗真菌药物、AgNP和ROS抗性的报告。我们的结果表明，体内念珠菌病是一个多因素的复杂过程，在提供对患者的有效治疗时，应综合考虑营养条件、人体免疫系统和病原体的适应性。
