Thoke Henrik Seir, Tobiesen Asger, Brewer Jonathan, Hansen Per Lyngs, Stock Roberto P, Olsen Lars F, Bagatolli Luis A
MEMPHYS-Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
MEMPHYS-Center for Biomembrane Physics, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK5230, Odense M, Denmark.
PLoS One. 2015 Feb 23;10(2):e0117308. doi: 10.1371/journal.pone.0117308. eCollection 2015.
We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.
我们检测到酿酒酵母糖酵解过程中ATP振荡浓度与细胞内水动力学之间存在非常强的耦合。我们的结果表明：i）细胞内水的偶极弛豫在细胞内是异质的，且与稀溶液条件不同；ii）水偶极弛豫随糖酵解振荡，并与ATP浓度同相；iii）从亚细胞到同步细胞群体，这种现象是尺度不变的；iv）糖酵解振荡和偶极弛豫的周期性均受到D2O的剂量依赖性同等影响。这些结果为细胞的一种新兴强度物理化学性质，即全细胞水偶极弛豫，与由强烈振荡的代谢过程产生的一种中心代谢物（ATP）之间的耦合提供了新的见解。
