Witte V, Grossmann B, Emeis C C
Institut für Mikrobiologie/Biologie IV, RWTH Aachen, Federal Republic of Germany.
Arch Microbiol. 1989;152(5):441-6. doi: 10.1007/BF00446926.
Random genomic DNA fragments from Kluyveromyces marxianus were cloned in order to identify chromosomal bands in pulsed field electrophoresis patterns of intergneric hybrid strains which were obtained by protoplast fusion. Molecular hybridization data indicated that the K. marxianus parental strain might be triploid, and it showed strong chromosome length polymorphism. We analyzed the karyotype of two Saccharomyces cerevisiae/K. marxianus hybrid strains (St. 1.St.46) with our DNA probes and with a Ty1 specific probe. We found indications for recombinational events which lead to the formation of hybrid chromosomal DNA molecules.
为了在通过原生质体融合获得的属间杂交菌株的脉冲场电泳图谱中识别染色体条带，对马克斯克鲁维酵母的随机基因组DNA片段进行了克隆。分子杂交数据表明，马克斯克鲁维酵母亲本菌株可能是三倍体，并且表现出强烈的染色体长度多态性。我们用DNA探针和Ty1特异性探针分析了两株酿酒酵母/马克斯克鲁维酵母杂交菌株（St.1、St.46）的核型。我们发现了导致杂交染色体DNA分子形成的重组事件的迹象。
