Marasini Bishnu P, Baral Pankaj, Aryal Pratibha, Ghimire Kashi R, Neupane Sanjiv, Dahal Nabaraj, Singh Anjana, Ghimire Laxman, Shrestha Kanti
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal ; Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal.
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal ; Department of Pathobiological Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Biomed Res Int. 2015;2015:265425. doi: 10.1155/2015/265425. Epub 2015 Feb 9.
The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range of in vitro growth inhibitory action against the tested bacterial species, and Cynodon dactylon was found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistant Staphylococcus aureus, imipenem-resistant Pseudomonas aeruginosa, multidrug-resistant Salmonella typhi, and S. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts of Cinnamomum camphora, Curculigo orchioides, and Curcuma longa exhibited the highest antibacterial activity against S. pyogenes with a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction of Cynodon dactylon exhibited best antibacterial activity against S. aureus with a MIC of 31 μg/mL. Among all, C. dactylon, C. camphora, C. orchioides, and C. longa plant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.
社区获得性和医疗保健相关细菌感染中多药耐药性在全球范围内的增加，削弱了当前的抗菌治疗效果，因此需要寻找其他替代方法。我们旨在使用微量肉汤稀释法，研究尼泊尔16种传统药用植物的乙醇提取物对13种临床细菌和2种参考细菌的体外抗菌活性。结果发现，所评估的植物物种对测试细菌物种具有一系列体外生长抑制作用，其中狗牙根对包括耐甲氧西林金黄色葡萄球菌、耐亚胺培南铜绿假单胞菌、多重耐药伤寒沙门氏菌和鼠伤寒沙门氏菌在内的13种细菌表现出中等抑制作用。测试乙醇提取物的最低抑菌浓度（MIC）值在31至>25,000μg/mL之间。值得注意的是，樟树、仙茅和姜黄的乙醇提取物对化脓性链球菌表现出最高的抗菌活性，MIC分别为49、49和195μg/mL；而狗牙根的氯仿馏分对金黄色葡萄球菌表现出最佳抗菌活性，MIC为31μg/mL。其中，狗牙根、樟树、仙茅和姜黄的植物提取物显示出MIC<100μg/mL的潜在抗菌活性。
