Department of Medicinal Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark.
BMC Complement Altern Med. 2012 Jun 14;12:74. doi: 10.1186/1472-6882-12-74.
Nearly 3,000 plant species are used as medicines in South Africa, with approximately 350 species forming the most commonly traded and used medicinal plants. In the present study, twelve South African medicinal plants were selected and tested for their antimicrobial activities against eight microbial species belonging to fungi, Mycobacteria, Gram-positive and Gram-negative bacteria.
The radiometric respiratory technique using the BACTEC 460 system was used for susceptibility testing against Mycobacterium tuberculosis, and the liquid micro-broth dilution was used for other antimicrobial assays.
The results of the minimal inhibitory concentration (MIC) determinations indicated that the methanol extracts from Acacia karoo, Erythrophleum lasianthum and Salvia africana were able to prevent the growth of all the tested microorganisms. All other samples showed selective activities. MIC values below 100 μg/ml were recorded with A. karoo, C. dentate, E. lasianthum, P. obligun and S. africana on at least one of the nine tested microorganisms. The best activity (MIC value of 39.06 μg/ml) was noted with S. africana against E. coli, S. aureus and M. audouinii, and Knowltonia vesitoria against M. tuberculosis.
The overall results of the present work provide baseline information for the possible use of the studied South African plant extracts in the treatment of microbial infections.
在南非，近 3000 种植物被用作药物，其中约 350 种是最常交易和使用的药用植物。在本研究中，选择了 12 种南非药用植物，并测试了它们对属于真菌、分枝杆菌、革兰氏阳性和革兰氏阴性细菌的 8 种微生物的抗菌活性。
使用 BACTEC 460 系统的放射性呼吸技术对结核分枝杆菌进行药敏试验，使用液体微量稀释法进行其他抗菌试验。
最小抑菌浓度（MIC）测定结果表明，卡拉哈里相思树、非洲藜芦和非洲鼠尾草的甲醇提取物能够阻止所有测试微生物的生长。所有其他样品均表现出选择性活性。卡拉哈里相思树、齿叶黄皮树、非洲藜芦、P. obligun 和非洲鼠尾草的甲醇提取物在至少一种测试的 9 种微生物中，MIC 值低于 100μg/ml。非洲鼠尾草对大肠杆菌、金黄色葡萄球菌和 M. audouinii 的活性最佳（MIC 值为 39.06μg/ml），而 Knowltonia vesitoria 对结核分枝杆菌的活性最佳。
本工作的总体结果为研究南非植物提取物在治疗微生物感染方面的可能用途提供了基线信息。
