Uberegui Estefanía, Hall Michael, Lorenzo Óscar, Schröder Wolfgang P, Balsera Mónica
Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), 37008-Salamanca, Spain Department of Chemistry, Umeå University, SE-90187 Umeå, Sweden.
Department of Chemistry, Umeå University, SE-90187 Umeå, Sweden.
J Exp Bot. 2015 Apr;66(7):2067-77. doi: 10.1093/jxb/erv018. Epub 2015 Mar 4.
The Executer1 and Executer2 proteins have a fundamental role in the signalling pathway mediated by singlet oxygen in chloroplast; nonetheless, not much is known yet about their specific activity and features. Herein, we have followed a differential-expression proteomics approach to analyse the impact of Executer on the soluble chloroplast protein abundance in Arabidopsis. Because singlet oxygen plays a significant role in signalling the oxidative response of plants to light, our analysis also included the soluble chloroplast proteome of plants exposed to a moderate light intensity in the time frame of hours. A number of light- and genotype-responsive proteins were detected, and mass-spectrometry identification showed changes in abundance of several photosynthesis- and carbon metabolism-related proteins as well as proteins involved in plastid mRNA processing. Our results support the participation of the Executer proteins in signalling and control of chloroplast metabolism, and in the regulation of plant response to environmental changes.
执行者1和执行者2蛋白在叶绿体中由单线态氧介导的信号通路中发挥着重要作用；然而，目前对它们的具体活性和特性了解还不多。在此，我们采用差异表达蛋白质组学方法来分析执行者对拟南芥可溶性叶绿体蛋白丰度的影响。由于单线态氧在植物对光的氧化反应信号传导中起重要作用，我们的分析还包括了在数小时时间范围内暴露于中等光照强度下的植物的可溶性叶绿体蛋白质组。检测到了许多光响应和基因型响应蛋白，质谱鉴定显示几种与光合作用和碳代谢相关的蛋白以及参与质体mRNA加工的蛋白丰度发生了变化。我们的结果支持执行者蛋白参与叶绿体代谢的信号传导和控制，以及植物对环境变化响应的调节。
