O群分枝杆菌噬菌体的比较基因组学
Comparative genomics of Cluster O mycobacteriophages.
作者信息
Cresawn Steven G, Pope Welkin H, Jacobs-Sera Deborah, Bowman Charles A, Russell Daniel A, Dedrick Rebekah M, Adair Tamarah, Anders Kirk R, Ball Sarah, Bollivar David, Breitenberger Caroline, Burnett Sandra H, Butela Kristen, Byrnes Deanna, Carzo Sarah, Cornely Kathleen A, Cross Trevor, Daniels Richard L, Dunbar David, Findley Ann M, Gissendanner Chris R, Golebiewska Urszula P, Hartzog Grant A, Hatherill J Robert, Hughes Lee E, Jalloh Chernoh S, De Los Santos Carla, Ekanem Kevin, Khambule Sphindile L, King Rodney A, King-Smith Christina, Klyczek Karen, Krukonis Greg P, Laing Christian, Lapin Jonathan S, Lopez A Javier, Mkhwanazi Sipho M, Molloy Sally D, Moran Deborah, Munsamy Vanisha, Pacey Eddie, Plymale Ruth, Poxleitner Marianne, Reyna Nathan, Schildbach Joel F, Stukey Joseph, Taylor Sarah E, Ware Vassie C, Wellmann Amanda L, Westholm Daniel, Wodarski Donna, Zajko Michelle, Zikalala Thabiso S, Hendrix Roger W, Hatfull Graham F
机构信息
Department of Biology, James Madison University, Harrisonburg, Virginia, United States of America.
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
出版信息
PLoS One. 2015 Mar 5;10(3):e0118725. doi: 10.1371/journal.pone.0118725. eCollection 2015.
Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages--Corndog, Catdawg, Dylan, Firecracker, and YungJamal--designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.
分枝杆菌噬菌体——分枝杆菌宿主的病毒——在基因上具有多样性，但在形态上都被归类于具有双链DNA和尾部的有尾噬菌体目。我们在此描述一组五个密切相关的分枝杆菌噬菌体——玉米热狗、猫热狗、迪伦、鞭炮和扬贾马尔——被指定为O簇，具有长而灵活的尾部，但有不寻常的长形衣壳。对噬菌体玉米热狗颗粒、猫热狗颗粒和受玉米热狗感染细胞的蛋白质组分析证实了一半预测基因产物的表达，并表明玉米热狗卷尺蛋白的翻译存在非经典机制。生物信息学分析确定了8 - 9个强烈预测的SigA启动子，并且所有五个O簇基因组在整个基因组中都包含超过30个具有二元对称的17 bp重复序列拷贝。O簇噬菌体的比较为噬菌体基因组进化提供了见解，包括水平基因交换导致的基因通量过程。
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