Rim Jeon Se, Ha Lee Keun, Ha Shin Dong, Sang Kwon Sun, Sung Hwang Jae
Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University.
J Gen Appl Microbiol. 2014;60(6):251-5. doi: 10.2323/jgam.60.251.
The antimicrobial activities of isolated compounds from seed extracts of Moringa oleifera and synergistic antimicrobial efficacy through hybridized complex of organic-inorganic composite materials were studied. The two main components of the Moringa oleifera seed were isolated and determined to be niazimicin and 4-(α-L-rhamnosyloxy)-benzyl isothiocyanate (RBI). The antimicrobial activity of the separated compounds of the Moringa oleifera seed were tested in vitro against 3 bacterial species and 2 fungal species by the paper disc diffusion assay and broth dilution methods. Both compounds showed antimicrobial activity against tested species and RBI was more effective than niazimicin. The MIC of RBI on S. aureus, E. coli, P. aeruginosa, C. albicans, and A. niger was 0.005%, 0.1%, 0.5%, 0.5%, and 0.5%, respectively, while the MIC of niazimicin on S. aureus was 0.1%. Next, we investigated the combined antimicrobial action of mesoporous ZnO and RBI by incorporating the compound within the pore of mesoporous ZnO. The MIC of mesoporous ZnO with RBI on S. aureus, E. coli, P. aeruginosa, C. albicans, and A. niger was 0.001%, 0.01%, 0.5%, 0.1%, and 0.1%, respectively. A synergistic effect of RBI with mesoporous ZnO was shown. From these results, the mesoporous ZnO could act as a reservoir for RBI and mesoporous ZnO with RBI could be used for cosmetic preservatives.
研究了辣木籽提取物中分离化合物的抗菌活性以及有机-无机复合材料杂交复合物的协同抗菌效果。分离并确定了辣木籽的两种主要成分，即尼亚齐米辛和4-(α-L-鼠李糖氧基)-苄基异硫氰酸酯（RBI）。通过纸片扩散法和肉汤稀释法，对辣木籽分离化合物的抗菌活性进行了体外测试，针对3种细菌和2种真菌。两种化合物均对测试菌种显示出抗菌活性，且RBI比尼亚齐米辛更有效。RBI对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌和黑曲霉的最低抑菌浓度（MIC）分别为0.005%、0.1%、0.5%、0.5%和0.5%，而尼亚齐米辛对金黄色葡萄球菌的MIC为0.1%。接下来，通过将RBI掺入介孔ZnO的孔内，研究了介孔ZnO与RBI的联合抗菌作用。介孔ZnO与RBI对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌和黑曲霉的MIC分别为0.001%、0.01%、0.5%、0.1%和0.1%。结果显示RBI与介孔ZnO具有协同作用。基于这些结果，介孔ZnO可作为RBI的储存库，介孔ZnO与RBI可用于化妆品防腐剂。
