Li Jinyao, Wang Xinhui, Wang Weilan, Luo JiaoJiao, Aipire Adila, Li Jinyu, Zhang Fuchun
College of Life Science and Technology, Xinjiang University, 14 Shengli Road, Urumqi 830046, Xinjiang, China.
College of Life Science and Technology, Xinjiang University, 14 Shengli Road, Urumqi 830046, Xinjiang, China.
Vaccine. 2015 Apr 15;33(16):1923-33. doi: 10.1016/j.vaccine.2015.02.063. Epub 2015 Mar 5.
Dendritic cells (DCs) play important roles in the regulation of immune system, which link innate and adaptive immune responses. Mature DCs produced interleukin (IL)-12 promote optimal type 1 T helper (Th1) cells and cytotoxic T lymphocytes. The extracts of traditional herbal medicines have been shown to enhance immune responses through promoting the maturation and cytokine production of DCs. Here, we investigated the effects of Pleurotus ferulae water extract (PFWE) on the maturation and function of bone marrow-derived DCs (BM-DCs). Upon PFWE treatment, BM-DCs dose-dependently upregulated the expression of CD40, CD80, CD86 and MHC II and increased the production of IL-12, IL-6 and tumor necrosis factor (TNF)-α but not for IL-10, which is mediated by TLR4 signaling pathway, at least partially. The production of prostaglandin E2 (PGE2) in BM-DCs was decreased by the treatment of PFWE. Moreover, PFWE treatment decreased the expression of active caspase-3 but increased the expression of CCR7. PFWE treated DCs enhanced the proliferation of allogenic CD8(+) T cells and the capacity of antigen presenting to autologous CD8(+) T cells. The combination of PFWE and CpG-ODN further enhanced the maturation and function of murine BM-DCs. The results showed that PFWE could enhance the maturation and function of DCs through TLR4 signaling pathway and has additive effect when combined with CpG-ODN, suggesting that PFWE alone or combined with CpG-ODN could be used to enhance the immune responses.
树突状细胞（DCs）在免疫系统调节中发挥重要作用，连接先天性和适应性免疫反应。成熟的DCs产生白细胞介素（IL）-12，促进最佳的1型辅助性T细胞（Th1）和细胞毒性T淋巴细胞的生成。传统草药提取物已被证明可通过促进DCs的成熟和细胞因子产生来增强免疫反应。在此，我们研究了阿魏菇水提取物（PFWE）对骨髓来源的DCs（BM-DCs）成熟和功能的影响。经PFWE处理后，BM-DCs剂量依赖性地上调CD40、CD80、CD86和MHC II的表达，并增加IL-12、IL-6和肿瘤坏死因子（TNF）-α的产生，但不增加IL-10的产生，这至少部分是由TLR4信号通路介导的。PFWE处理可降低BM-DCs中前列腺素E2（PGE2）的产生。此外，PFWE处理降低了活性半胱天冬酶-3的表达，但增加了CCR7的表达。PFWE处理的DCs增强了同种异体CD8(+) T细胞的增殖以及向自体CD8(+) T细胞呈递抗原的能力。PFWE与CpG-ODN联合进一步增强了小鼠BM-DCs的成熟和功能。结果表明，PFWE可通过TLR4信号通路增强DCs的成熟和功能，与CpG-ODN联合时有相加作用，提示PFWE单独或与CpG-ODN联合可用于增强免疫反应。
