Dolete Georgiana, Ilie Cornelia-Ioana, Chircov Cristina, Purcăreanu Bogdan, Motelica Ludmila, Moroșan Alina, Oprea Ovidiu Cristian, Ficai Denisa, Andronescu Ecaterina, Dițu Lia-Mara
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.
National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.
Gels. 2023 Apr 2;9(4):295. doi: 10.3390/gels9040295.
The aim of the present study was to obtain a hydrogel-based film as a carrier for the sustained and controlled release of vancomycin, an antibiotic commonly used in various types of infections. Considering the high-water solubility of vancomycin (>50 mg/mL) and the aqueous medium underlying the exudates, a prolonged release of vancomycin from an MCM-41 carrier was sought. The present work focused on the synthesis of malic acid coated magnetite (FeO/malic) by co-precipitation, synthesis of MCM-41 by a sol-gel method and loading of MCM-41 with vancomycin, and their use in alginate films for wound dressing. The nanoparticles obtained were physically mixed and embedded in the alginate gel. Prior to incorporation, the nanoparticles were characterized by XRD, FT-IR and FT-Raman spectroscopy, TGA-DSC and DLS. The films were prepared by a simple casting method and were further cross-linked and examined for possible heterogeneities by means of FT-IR microscopy and SEM. The degree of swelling and the water vapor transmission rate were determined, considering their potential use as wound dressings. The obtained films show morpho-structural homogeneity, sustained release over 48 h and a strong synergistic enhancement of the antimicrobial activity as a consequence of the hybrid nature of these films. The antimicrobial efficacy was tested against , two strains of (including vancomycin-resistant Enterococcus, VRE) and . The incorporation of magnetite was also considered as an external triggering component in case the films were used as a magneto-responsive smart dressing to stimulate vancomycin diffusion.
本研究的目的是获得一种基于水凝胶的薄膜，作为万古霉素（一种常用于各类感染的抗生素）持续控释的载体。考虑到万古霉素的高水溶性（>50 mg/mL）以及渗出液所处的水性介质，人们寻求从MCM-41载体中实现万古霉素的长效释放。本工作聚焦于通过共沉淀法合成苹果酸包覆的磁铁矿（FeO/苹果酸），通过溶胶 - 凝胶法合成MCM-41，并将万古霉素负载于MCM-41中，以及它们在用于伤口敷料的藻酸盐薄膜中的应用。所获得的纳米颗粒进行物理混合并嵌入藻酸盐凝胶中。在掺入之前，通过X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和傅里叶变换拉曼光谱（FT-Raman）、热重 - 差示扫描量热法（TGA-DSC）和动态光散射（DLS）对纳米颗粒进行表征。薄膜通过简单的流延法制备，进一步交联，并通过傅里叶变换红外显微镜和扫描电子显微镜（SEM）检查可能存在的不均匀性。考虑到它们作为伤口敷料的潜在用途，测定了溶胀度和水蒸气透过率。所获得的薄膜呈现出形态结构均匀性，48小时内持续释放，并且由于这些薄膜的混合性质，抗菌活性有强烈的协同增强。针对两种 菌株（包括耐万古霉素肠球菌，VRE）和 测试了抗菌效果。在薄膜用作磁响应智能敷料以刺激万古霉素扩散的情况下，磁铁矿的掺入也被视为一种外部触发成分。
